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MUG

MUG (4-MU-glcUA; 4-Methylumbelliferyl-β-D-glucuronic acid dihydrate), also known as MUGlcU, is a fluorogenic β-glucuronidase substrate that releases the blue fluorescent 4-methyl-7-hydroxycoumarinon enzymatic hydrolysis. The substrate is commonly used for identifying E. coli contamination and for detecting marker GUS gene expression in plants with high sensitivity.

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CAS number

6160-80-1

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Product Description

MUG (4-MU-glcUA; 4-Methylumbelliferyl-β-D-glucuronic acid dihydrate), also known as MUGlcU, is a fluorogenic β-glucuronidase substrate that releases the blue fluorescent 4-methyl-7-hydroxycoumarinon enzymatic hydrolysis. The substrate is commonly used for identifying E. coli contamination and for detecting marker GUS gene expression in plants with high sensitivity.

  • λ ExEm= 360/450 nm (end product)
  • White solid soluble in DMSO
  • Store desiccated at ≤4°C and protect from light
  • C16H16O9.2H2O
  • MW: 388.3
  • [6160-80-1]

 

References

1. BioTechniques 8, 39 (1990).

2. Plant Mol. Biol. Rep. 5, 387 (1987).

 

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    X-GlcU, Na

    X-GlcU, Na ( 5-Bromo-4-chloro-3-indolyl-β-D-glucuronide, sodium salt), also known as X-GlcA, Na, is similar to X-GlcU, CHA, but has better water solubility.

    Red-β-D-GlcU,CHA

    Red-β-D-GlcU (5-Bromo-6-chloro-3-indolyl-β-D-glucuronide,cyclohexylammmonium salt), also known as Magenta-β-D-GlcA; is similar to X-GlcU, but the product of the substrate is a red (λmax 565 nm) precipitate, which is easier to detect against the background of plant cells.

    Rose-β-D-GlcU,CHA

    Rose-β-D-GlcU (6-Chloro-3-indolyl-β-D-glucuronide,cyclohexylammonium salt), also known as Salmon-β-D-GlcUA, is similar to X-GlcU, but the product of the substrate is a rose-colored (λmax ~ 540 nm) precipitate, which is easier to detect against the background of plant cells.

    X-GlcU, CHA

    Alternate name: BCIG. X-GlcU (5-Bromo-4-chloro-3-indolyl-β-D-glucuronide, cyclohexylammonium salt) is a chromogenic β-glucuronidase substrate widely used for monitoring E. coli contamination in food (such as meat, dairy products and shellfish, etc) and water, and for the detection of GUS gene expression in plants.