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Rose-β-D-Gal

Rose-β-D-Gal (6-Chloro-3-indoxyl-β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase. The product is similar to X-gal, but generates an intense pink colored precipitate (λmax ~ 540 nm) on enzymatic hydrolysis.

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Product Description

Rose-β-D-Gal (6-Chloro-3-indoxyl-β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase. The product is similar to X-gal, but generates an intense pink colored precipitate (λmax ~ 540 nm) on enzymatic hydrolysis.

  • White solid soluble in DMSO
  • Store at 4°C and protect from light
  • C14H16ClNO6

 

References

1. Molecular Cloning: A laboratory Manual, Cold Spring Harbor Laboratory, B. 14, p186 (1989).

2. Biotechniques 27, 438 (1999).

3. Oncogene 10, 2323 (1995).

4. Dev Biol 161, 77 (1994).

5. J Histochem Cytochem 42, 1299 (1994).

6. BioTechniques 15, 974 (1993).

7. BioTechniques, 15, 292 (1993).

 

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    Chromogenic β-Galactosidase Substrate Sampler

    For researchers wishing to investigate the suitability of our various chromogenic β-galactosidase substrates, we offer this sampler kit, which contains 5 mg each of the following five different substrates: X-Gal (10011), Red-β-D-Gal (10012), Rose-β-D-Gal (10013), Purple-β-D-Gal (10014) and Green-β-D-Gal (10015).

    X-Gal

    X-gal (5-Bromo-4-chloro-3-indolyl-β-galactopyranoside) is the most widely used chromogenic substrate for β-galactosidase. Enzyme hydrolysis of the substrate yields a dark blue colored precipitate (maximum absorbance 615 nm).

    Red-β-D-Gal

    Red-β-D-Gal (5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase. Similar to X-gal, but generates a red or magenta (λmax ~ 565 nm) colored precipitate on enzyme hydrolysis.

    Purple-β-D-Gal

    Purple-β-D-Gal (5-Iodo-3-indolyl-β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase. Similar to X-gal, but generates a purple (λmax ~575 nm) colored precipitate on enzyme hydrolysis.

    Green-β-D-Gal

    Green-β-D-Gal (N-Methylindolyl-β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase. Similar to X-Gal, but generates green colored precipitate on enzyme hydrolysis.