Red-β-D-Gal (5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase. Similar to X-gal, but generates a red or magenta (λmax ~ 565 nm) colored precipitate on enzyme hydrolysis.
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Chromogenic β-Galactosidase Substrate Sampler
For researchers wishing to investigate the suitability of our various chromogenic β-galactosidase substrates, we offer this sampler kit, which contains 5 mg each of the following five different substrates: X-Gal (10011), Red-β-D-Gal (10012), Rose-β-D-Gal (10013), Purple-β-D-Gal (10014) and Green-β-D-Gal (10015).
X-gal (5-Bromo-4-chloro-3-indolyl-β-galactopyranoside) is the most widely used chromogenic substrate for β-galactosidase. Enzyme hydrolysis of the substrate yields a dark blue colored precipitate (maximum absorbance 615 nm).
Rose-β-D-Gal (6-Chloro-3-indoxyl-β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase. The product is similar to X-gal, but generates an intense pink colored precipitate (λmax ~ 540 nm) on enzymatic hydrolysis.
Purple-β-D-Gal (5-Iodo-3-indolyl-β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase. Similar to X-gal, but generates a purple (λmax ~575 nm) colored precipitate on enzyme hydrolysis.