Support & Resources

+1

Zinquin free acid

Zinquin is an UV-excitable, blue fluorescent zinc indicator. Zinc is believed to be involved in the suppression of apoptosis and thought to play important roles in many neural activities.

Size
Catalog #
price
Qty
5 mg
Clear selection
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Protocols
SDS

Product Description

Zinquin is an UV-excitable, blue fluorescent zinc indicator. Zinc is believed to be involved in the suppression of apoptosis and thought to play important roles in many neural activities.

  • λExEm (with Zn2+)= 364/485 nm
  • Off-white solid soluble in DMSO
  • Store at 4°C and protect from light
  • C19H18N2O5S
  • MW: 386.42

As the indicator does not covalently bind to cellular components, it may be actively effluxed from the cell by organic anion transporters. The rate of efflux increases with temperature, and may vary between cell types, resulting in variable retention times of a few minutes to hours. Experiments using indicators in cells usually are performed within one or two hours of loading, but it may be possible to re-load cells with indicator if needed. The organic anion transporter inhibitor Probenecid (#50027) can be used to slow the rate of indicator efflux from cells.

References

  1. Biochem J, 303, 781 (1994), DOI: 10.1042/bj3030781
  2. Biochem J, 296, 403 (1993), DOI: 10.1042/bj2960403
  3. Methods Cell Biol, 99, 113, (2021), DOI: 10.1016/B978-0-12-374841-6.00005-0
 

You may also like…

    MQAE

    MQAE (N-(Ethoxycarbonylmethyl)-6-methoxyquinolinium bromide) is an improved chloride indicator that has greater sensitivity to chloride than SPQ, and higher fluorescence quantum yield.

    Zinquin ethyl ester

    Zinquin is an UV-excitable, blue fluorescent zinc indicator. Zinquin ethyl ester is membrane-permeable and is hydrolyzed into Zinquin free acid once entering cells.

    SPQ

    The fluorescence of SPQ is specifically quenched by chloride via collision. Therefore, chloride concentration is measured by monitoring the degree of fluorescence decrease.

    BCECF, AM Ester, 1 mg/mL in Anhydrous DMSO

    BCECF, AM ester is membrane-permeant and thus can be loaded into cells via incubation. This product is a mixture of three molecular species, all of which are readily hydrolyzed by endogenous esterases into a single BCECF free acid form once they are in the cells.

    MitoView™ Mitochondrial Dyes

    Fluorogenic mitochondrial stains for live cells that rapidly accumulate in mitochondria and can be imaged without washing.