Probenecid, Sodium Salt, Water Soluble
Probenecid is commonly used to inhibit organic-anion transporters located in the cell membrane. Such transporters can extrude dyes and indicators and thus contribute to low signal or high background in assays due to poor cellular retention of dyes.
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BAPTA, AM Ester
BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.
4-Bromo A-23187, Free Acid
Similar to A-23187, 4-Bromo A-23187 is used for in situ calibrations of fluorescent Ca2+ indicators to equilibrate intracellular and extracellular calcium concentrations and to allow Mn2+ to enter cells to quench indicator fluorescence.
Fluo-3, AM Ester
Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate dye loading.
Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation.