RNA & DNA EXTRACTION KITS

Formalin-fixed tissue samples are a challenge for RNA and DNA extraction, often resulting in low yields and poor performance in subsequent steps. Most existing methods rely on heat to remove crosslinks and adducts, which is only partially effective and leads to additional fragmentation of labile nucleic acids. In contrast, the catalytic CAT5™ technology, built on research initiated at Stanford University, greatly accelerates the removal of formaldehyde damage and allows recovery of higher quality nucleic acids. This results in higher yields, more intact RNA or DNA, and better results in downstream analysis like PCR, microarray, or next-generation sequencing (NGS).

RNAstorm™ Kit for Isolation of RNA from FFPE Tissue Samples

Powered by proprietary CAT5™ technology, the RNAstorm™ FFPE isolation kit enhances the removal of formaldehyde-induced damage and purifies RNA with higher yield and quality, better integrity, and greater amplifiability.
The superior quality of RNAstorm-derived RNA has been demonstrated in many ways, including higher RIN score, higher yield, higher DV200, and improved RNA-seq alignment. According to a tech note from Illumina, the best predictor of successful performance in Illumina RNA-seq is the DV200 score, which represents the percentage of RNA fragments longer than 200 nucleotides. Whether you are performing RNA-seq, RT-qPCR, microarray, or other gene expression analysis, the RNAstorm™ kit is your best chance for success.

Peer-reviewed studies demonstrate the RNAstorm™ FFPE RNA Extraction Kit yields high amounts of quality RNA for diverse applications.

Sign up to our e-newsletter to download our Literature Digest: Higher Quality and Yields of FFPE RNA for Diverse Applications with RNAstorm™. Featuring publications that showcase the diverse fields of research and downstream workflows in which RNAstorm™ has been used, including data on improved RNA yields and quality versus other commercially available kits.

Purify high quality total RNA from either cultured cells or fresh tissue in as little as 20 minutes. High yields (up to 120 μg) can be obtained using a simple and quick column-based protocol. Contaminating DNA is removed using a DNase treatment step, and the protocol avoids toxic chemicals such as phenol or chloroform.

Advantages of the RNAstorm™ RNA Isolation Kit

• High quality total RNA in as little as 20 minutes
• Simple workflow yielding up to 120 μg of RNA
• DNase treatment included
• No phenol-chloroform and no ethanol precipitation
• Ready to use for next-generation sequencing (RNA-Seq), RT-PCR, cDNA synthesis, and microarrays

Efficient total RNA extraction from purified EVs

The ExoBrite™ EV Total RNA Isolation Kit was designed to address the challenges of EV RNA extraction by offering an optimized and easy-to-use kit for total RNA isolation, including mRNA and miRNAs, from purified EVs. The isolated EV RNA can then be used for downstream analysis such as qPCR or RNAseq.

Advantages of ExoBrite™ EV Total RNA Isolation Kit:

• Optimized for total RNA extraction from purified EVs
• Recover ~10-20 ng of RNA from 1×10¹⁰ SEC-enriched EVs
• Compatible with downstream applications such as qPCR or RNAseq
• Simple column-based purification
• No phenol/chloroform or ethanol precipitation steps

Biotium’s DNA Gel Extraction Kit is a silica-gel spin column based DNA extraction kit designed to purify 40 bp-40 kb DNA fragments from agarose gels in TAE or TBE buffer. Each column has a maximum binding capacity of 10 ug DNA. Biotium’s DNA Gel Extraction Kit removes GelRed™ and GelGreen™ Nucleic Acid Gel Stains, along with other commonly used nucleic acid gel stains. The purified DNA is compatible with common downstream applications such as transformation, sequencing, PCR, and restriction digests.