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CELLDATA DNAstorm™ 2.0 FFPE DNA Extraction Kit

Superior DNA extraction from FFPE samples, due to the enhanced removal of formaldehyde-induced damage, providing DNA with higher yield and quality and greater amplifiability.

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Formalin-fixed tissue samples are a challenge for DNA extraction, often resulting in low yields and poor performance in subsequent steps. Most existing methods rely on heat to remove crosslinks and adducts, which is only partially effective and leads to additional fragmentation of labile nucleic acids. Powered by proprietary CAT5™ technology, the DNAstorm™ 2.0 FFPE Extraction Kit enhances the removal of formaldehyde-induced damage and provides DNA with higher yield and quality and greater amplifiability. The DNAstorm™ 2.0 FFPE DNA Extraction Kit is the best solution for next-generation sequencing and other advanced applications.

  • Purify higher quality DNA with less fragmentation
  • Extract higher yields of amplifiable DNA compared to other methods
  • CAT5™ technology for chemical reversal of formaldehyde crosslinking
  • Milder conditions and no harsh solvents
  • Better results in downstream analysis like PCR, microarray, or next-generation sequencing
  • Simple workflow

Note: This kit is a replacement for catalog number CD502. It uses a modified deparaffinization protocol with a new Dewaxing Solution (provided in the kit). The new kit retains the same proprietary CAT5™ catalytic technology for highly efficient reversal of formaldehyde crosslinks as the original kit.

The DNAstorm™ 2.0 DNA  extraction kit greatly accelerates the removal of formaldehyde damage and allows recovery of higher quality nucleic acids. This results in higher yields, more intact DNA, and better results in downstream analysis like PCR, microarray, or next-generation sequencing (NGS). See the product table below for our complete line of RNAstorm™ and DNAstorm™ extraction kits for FFPE tissues.

DNA from four different FFPE tumor samples (colorectal, lung, bladder, and esophagus) was extracted using the DNAstorm™ FFPE kit along with a popular competitor’s kit. Equal amounts (500 ng) of DNA were loaded and run on a pulsed field gel. Significant improvements in average DNA size are seen using the DNAstorm™ kit. “Kit R” represents a competitive commercial DNA FFPE extraction kit.

RNAstorm™ and DNAstorm™ FFPE Extraction Kits

Product NameCatalog NumberSize
CELLDATA RNAstorm™ 2.0 FFPE RNA Extraction KitCD50650 preps
CELLDATA DNAstorm™ 2.0 FFPE DNA Extraction KitCD50750 preps
CELLDATA DNAstorm™/RNAstorm™ 2.0 Combination KitCD50850 preps
CELLDATA RNAstorm™ 2.0 MagBead FFPE RNA Extraction KitCD510-9696 preps
CELLDATA DNAstorm™ 2.0 MagBead FFPE DNA Extraction KitCD509-9696 preps

References

Download a list of Mix-n-Stain™ references.

 

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