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RNA & DNA Isolation from FFPE Tissue Samples
CAT5™ Catalytic Technology
Formalin-fixed tissues often yield poor quality RNA/DNA due to crosslinks and adducts. Extraction methods that use heat can cause further fragmentation. CAT5™ technology uses a catalytic approach to efficiently remove formaldehyde damage, improving nucleic acid recovery, integrity, and performance in PCR, microarray, or NGS.
- Chemical reversal of formaldehyde crosslinking
- Effective: Higher yields compared to other methods
- Gentle: Milder conditions and no harsh solvents
- Higher quality RNA or DNA with less fragmentation
RNAstorm™ Kit for Isolation of RNA from FFPE
Powered by proprietary CAT5™ technology, the RNAstorm™ FFPE isolation kit enhances the removal of formaldehyde-induced damage and purifies RNA with higher yield and quality, better integrity, and greater amplifiability. The superior quality of RNAstorm-derived RNA has been demonstrated in many ways, including higher RIN score, higher yield, higher DV200, and improved RNA-seq alignment.
DNAstorm™ Kit for Isolation of DNA from FFPE
Read Peer-Reviewed Studies Validating RNAstorm™
Literature Digest
Literature Digest: Higher Quality and Yields of FFPE RNA for Diverse Applications with RNAstorm™
RNAstorm™ and DNAstorm™ FFPE Extraction Kits
| Product Name | Catalog Number | Size |
|---|---|---|
| CELLDATA RNAstorm™ 2.0 FFPE RNA Extraction Kit | CD506 | 50 preps |
| CELLDATA DNAstorm™ 2.0 FFPE DNA Extraction Kit | CD507 | 50 preps |
| CELLDATA DNAstorm™/RNAstorm™ 2.0 Combination Kit | CD508 | 50 preps |
| CELLDATA RNAstorm™ 2.0 MagBead FFPE RNA Extraction Kit | CD510-96 | 96 preps |
| CELLDATA DNAstorm™ 2.0 MagBead FFPE DNA Extraction Kit | CD509-96 | 96 preps |
RNA Isolation from Fresh Tissue and Cells
Purify high quality total RNA from either cultured cells or fresh tissue in as little as 20 minutes. High yields (up to 120 μg) can be obtained using a simple and quick column-based protocol. Contaminating DNA is removed using a DNase treatment step, and the protocol avoids toxic chemicals such as phenol or chloroform.
- High quality total RNA in as little as 20 minutes
- Simple workflow yielding up to 120 μg of RNA
- DNase treatment included
- No phenol-chloroform and no ethanol precipitation
- Ready to use for next-generation sequencing (RNA-Seq), RT-PCR, cDNA synthesis, and microarrays
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EV Total RNA Extraction
Efficient Total RNA Extraction from Purified EVs
The ExoBrite™ EV Total RNA Isolation Kit was designed to address the challenges of EV RNA extraction by offering an optimized and easy-to-use kit for total RNA isolation, including mRNA and miRNAs, from purified EVs. The isolated EV RNA can then be used for downstream analysis such as qPCR or RNAseq.
Explore our full selection of unique stains and antibodies for EV detection.
- Optimized for total RNA extraction from purified EVs
- Recover ~10-20 ng of RNA from 1×1010 SEC-enriched EVs
- Compatible with downstream applications such as qPCR or RNAseq
- Simple column-based purification
- No phenol/chloroform or ethanol precipitation steps
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DNA Gel Extraction Kit
Biotium’s DNA Gel Extraction Kit is a silica-gel spin column based DNA extraction kit designed to purify 40 bp-40 kb DNA fragments from agarose gels in TAE or TBE buffer. Each column has a maximum binding capacity of 10 ug DNA. Biotium’s DNA Gel Extraction Kit removes GelRed® and GelGreen® Nucleic Acid Gel Stains, along with other commonly used nucleic acid gel stains. The purified DNA is compatible with common downstream applications such as transformation, sequencing, PCR, and restriction digests.
