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5X Firefly Luciferase Lysis Buffer

5X Firefly Lysis Buffer is a concentrated cell lysis buffer that is designed for use with Biotium’s Firefly Luciferase Assay Kit  and Firefly Luciferase Assay Kit (Lyophilized).

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Product Description

5X Firefly Lysis Buffer is a concentrated cell lysis buffer that is designed for use with Biotium’s Firefly Luciferase Assay Kit 2.0 (catalog no. 30085) and Firefly Luciferase Assay Kit (Lyophilized) (catalog no. 30075). These kits are supplied with sufficient 5X Firefly Lysis Buffer to perform the stated number of assays with cells grown in 96 – 24 well plates. 5X Firefly Lysis Buffer is sold separately for use with the kits when more lysis buffer (e.g. >100 uL/well) is required.

Note: 5X Firefly Lysis Buffer is not compatible with Renilla Luciferase. If you require additional lysis buffer for use with Biotium’s Renilla Luciferase Assay Kit 2.0 (catalog no. 30004) or Firefly and Renilla Luciferase Single Tube Assay Kit (catalog no. 30081), order 5X Passive Lysis Buffer (catalog no. 99912).

More Luciferase Kits:

Catalog numberProductDescription
30085Firefly Luciferase Assay Kit 2.0Flash-type firefly luciferase assay kit.
30075Firefly Luciferase Assay Kit (Lyophilized)Firefly luciferase assay kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C.
999235X Firefly Lysis BufferExtra lysis buffer for use with Firefly Luciferase Assay Kits, only required if using well sizes larger than 24-well.
30028Steady-Luc™ Firefly HTS Assay KitGlow-type firefly luciferase assay with signal half-life of about 3 hours for high-throughput screening in multi-well plates.
30028LSteady-Luc Firefly HTS Assay Kit (Lyophilized)Steady-Luc kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C.
30082Renilla Luciferase Assay Kit 2.0Flash-type Renilla luciferase assay kit.
999125X Passive Lysis BufferExtra lysis buffer for use with Renilla Luciferase Assay Kits, only required if using well sizes larger than 24-well.
30081Firefly and Renilla Luciferase Single Tube Assay KitAssay kit for measuring firefly and Renilla activities sequentially in the same sample in a single tube.
99821-100mL1X Passive Lysis Buffer 2.0 Extra lysis buffer for use with Firefly & Renilla Single Tube Assay Kits, only required if using well sizes larger than 24-well.
30020ATP-Glo™ Bioluminometric Cell Viability Assay KitFlash-type luminescence-based assay for measuring cell viability or ATP concentration.
22003Mini Cell ScrapersMini-cell scrapers, useful for lysing cells in 96-well, 48-well, or 24-well plates.

References

  1. PLoS ONE (2011), doi:10.1371/journal.pone.0025858
  2. British J Pharmacol. (2014), doi: 10.1111/bph.13014
  3. J Nutritional Biochem. (2013), https://www.sciencedirect.com/science/article/pii/S0955286313001435
  4. J Biol. Chem. 291, 110 (2015).
 

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