5X Firefly Luciferase Lysis Buffer

Most of our products are stable at room temperature for many days, so in all likelihood the product will still work just fine. To be on the safe side, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.

One exception that we are aware of is GelGreen™, which is more sensitive to light exposure than most of our other fluorescent dyes. If GelGreen™ is exposed to ambient light for a prolonged period of time (days to weeks), its color will change from dark orange to brick red. If this occurs, the GelGreen will no longer work for gel staining.

The ATP-Glo™ Bioluminometric Cell Viability Assay is a luciferase-based assay for quantifying cellular ATP. The kit is a flash-type assay with a signal stability of 1 minute, and therefore requires a microplate reader equipped with reagent injectors for high-throughput (HTS) screening.

The Steady-ATP™ HTS Viability Assay Kit is a glow-type assay that serves as an improved replacement for the ATP-Glo™ kit. The kit offers a half-life longer than 5 hours and a linear signal over 3-4 orders of magnitude. The extended half-life offers greater flexibility for HTS platforms over the ATP-Glo™ kit with comparable sensitivity.

Some of Biotium’s luciferase assay kits can be used as alternatives to Promega kits. The comparable products are listed below. Click on a link for product information.