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1X Passive Lysis Buffer 2.0

Extra lysis buffer for use with Firely & Renilla Luciferase Single Tube Assay Kits.

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Store at -10 to -35 °C

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100 mL
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Product Description

1X Passive Lysis Buffer 2.0 is a cell lysis buffer that is designed for use with Biotium’s Firefly & Renilla Luciferase Single Tube Assay Kit (30081). Those kits are supplied with sufficient lysis buffer to perform the stated number of assays with cells grown in 96 – 24 well plates. 1X Passive Lysis Buffer 2.0 is sold separately for use with the kits when more lysis buffer (e.g. >100 uL/well) is required.

  • Additional 1X Passive Lysis Buffer 2.0 for larger volume assays
  • For use with Biotium’s Firefly & Renilla Luciferase Single Tube Assay Kit (30081)

Oure kits are supplied with sufficient lysis buffer to perform the stated number of assays with cells grown in 96 – 24 well plates. 1X Passive Lysis Buffer 2.0 is sold separately for use with the kits when more lysis buffer (e.g. >100 uL/well) is required.

This buffer also can also be used to dilute recombinant firefly or Renilla luciferase enzymes without the need to add BSA to stabilize the enzymes.

Note: If additional lysis buffer is required for the Firefly Luciferase Assay Kit 2.0 (30085) or the Firefly Luciferase Assay (Lyophilized) (30075), purchase 5X Firefly Luciferase Lysis Buffer (99923). If additional lysis buffer is required for Renilla Luciferase Assay Kit 2.0 (30082), purchase 5X Passive Lysis Buffer (99912).

More Luciferase Kits

Catalog number Product Description
30085 Firefly Luciferase Assay Kit 2.0 Flash-type firefly luciferase assay kit.
30075 Firefly Luciferase Assay Kit (Lyophilized) Firefly luciferase assay kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C.
99923 5X Firefly Lysis Buffer Extra lysis buffer for use with Firefly Luciferase Assay Kits, only required if using well sizes larger than 24-well.
30028 Steady-Luc™ Firefly HTS Assay Kit Glow-type firefly luciferase assay with signal half-life of about 3 hours for high-throughput screening in multi-well plates.
30028L Steady-Luc Firefly HTS Assay Kit (Lyophilized) Steady-Luc kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C.
30082 Renilla Luciferase Assay Kit 2.0 Flash-type Renilla luciferase assay kit.
99912 5X Passive Lysis Buffer Extra lysis buffer for use with Renilla Luciferase Assay Kits, only required if using well sizes larger than 24-well.
30081 Firefly and Renilla Luciferase Single Tube Assay Kit Assay kit for measuring firefly and Renilla activities sequentially in the same sample in a single tube.
99821-100mL 1X Passive Lysis Buffer 2.0 Extra lysis buffer for use with Firefly & Renilla Single Tube Assay Kits, only required if using well sizes larger than 24-well.
30020 ATP-Glo™ Bioluminometric Cell Viability Assay Kit Flash-type luminescence-based assay for measuring cell viability or ATP concentration.
22003 Mini Cell Scrapers Mini-cell scrapers, useful for lysing cells in 96-well, 48-well, or 24-well plates.
 

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