Support & Resources

+1

ABD-F

ABD-F is a useful reagent for the sensitive and specific detection of thiols both by TLC and HPLC (1,2). When combined with a reducing agent such as tributylphosphine, it can also be used for the determination of disulfides in peptides and proteins (3,4).

Product Attributes

Excitation/Emission

375/508 nm

Learn more about

Technology

Size
Catalog #
price
Qty
10 mg
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Protocols
SDS

Product Description

ABD-F is a useful reagent for the sensitive and specific detection of thiols both by TLC and HPLC (1,2). When combined with a reducing agent such as tributylphosphine, it can also be used for the determination of disulfides in peptides and proteins (3,4).

  • λExEm(N-acetylcysteine conjugate at pH 2) = 375/508 nm
  • Of-white solid soluble in DMSO or DMF
  • Store desiccated at 4°C and protect from light especially in solution
  • C7H7FN3O3S
  • MW: 232

References

1. Anal Chem 56, 2461 (1984).

2. J Pharm Biomed Anal 24, 1137 (2001).

 

You may also like…

    DMSO, Anhydrous

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

    TCEP

    TCEP hydrochloride is an odorless reducing agent that is more stable and effective than dithiothreitol (DTT) or 2-Mercaptoethanol. TCEP hydrochloride is soluble in water to 310 grams per liter.

    DTT

    DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.

    CF® Dye Maleimides

    CF® Dye Maleimides are thiol-reactive fluorescent dyes. Maleimides are commonly used to label proteins, peptides or other molecules containing free thiol groups.

    CF® Dye MTS

    CF® dye MTS are fast-reacting, highly selective thiol-reactive derivatives for reversible labeling of thiol groups with fluorescent CF® dyes.

    MTS-BCN

    MTS-BCN is a fast-reacting and highly selective thiol-reactive label for attaching BCN to thiol groups on biomolecules. BCN, an alternative to DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition.