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BCIP Pink/NBT Kit

NBT and BCIP PINK are widely used together as a chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization. This kit contains 100 mg each of BCIP Pink (10006) and the precipitation inducer NBT (10008).

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Product Description

BCIP Pink (6-Chloro-3-indoxyl phosphate, p-toluidine salt) is a derivative of BCIP. While BCIP yields a blue precipitating product, BCIP Pink produces a pink colored (λmax 540 nm) precipitate. BCIP and the derivatives are the most widely used chromogenic phosphatase substrate. They are often used with the oxidant NBT (nitro blue tetrazolium chloride), which facilitates the precipitation, to detect alkaline phosphatase activity and alkaline phosphatase labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization.

  • Pink colormetric detection of alkaline phosphatase activity and labels
  • Compatible with a variety of  applications
  • Use BCIP Pink alone or in combination with NBT
  • White solid soluble in DMF

This kit contains 100 mg each of BCIP Pink (10006) and the precipitation inducer NBT (10008). We also offer Alkaline Phosphatase Conjugated Antibodies.

Find the Right Stain for your Application

The original BCIP forms a dark blue (λmax 615 nm) precipitate and is available in two different salt formulations; BCIP, toluidine salt is soluble in DMF while BCIP, sodium salt is soluble in water. We also offer a Pink BCIP derivative, which produces a pink colored (λmax 540 nm) precipitate. BCIP Red produces a red colored (λmax 565 nm) precipitate. Please see our BCIP Kits that are paired with NBT (nitro blue tetrazolium chloride) for user convenience.

 

 

 

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    BCIP, Na

    A water soluble formulation of the most widely used chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization.

    BCIP Red/NBT Kit

    A red derivative of BCIP that produces a red (λmax 565 nm) colored precipitate instead of the dark blue precipitate of BCIP. BCIP and derivatives are the most widely used chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications.

    BCIP/NBT Kit

    BCIP/NBT are widely used together as a chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization. This kit contains 100 mg each of BCIP sodium salt (10001) and the oxidant NBT (Nitro blue tetrazolium, 10008).

    Chromogenic β-Galactosidase Substrate Sampler

    For researchers wishing to investigate the suitability of our various chromogenic β-galactosidase substrates, we offer this sampler kit, which contains 5 mg each of the following five different substrates: X-Gal (10011), Red-β-D-Gal (10012), Rose-β-D-Gal (10013), Purple-β-D-Gal (10014) and Green-β-D-Gal (10015).

    Chromogenic Phosphatase Substrate Sampler

    The sampler kit contains 10 mg each of BCIP Na (10001), BCIP Pink (10006), BCIP Red (10004) and 250 mg of NBT (10008). NBT forms a co-precipitant and enhances the BCIP reaction.