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BCIP Pink

A pink derivative of BCIP that produces a pink colored (λmax 540 nm) precipitate instead of the dark blue precipitate of BCIP. BCIP and derivatives are the most widely used chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins and are compatible with a variety of applications.

Product Attributes

CAS number

159954-33-3

Molecular weight

354.7

Storage Conditions

Store at 2 to 8 °C or below, Protect from light, Desiccate

Applications

Microscopy, Immunohistology, Western, In situ hybridization, Tissue staining

Chemical Formula: C15H17ClN2O4P

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

BCIP Pink (6-Chloro-3-indoxyl phosphate, p-toluidine salt) is a derivative of BCIP. While BCIP yields a blue precipitating product, BCIP Pink produces a pink colored (λmax 540 nm) precipitate. BCIP and the derivatives are the most widely used chromogenic phosphatase substrate. They are often used with the oxidant NBT (nitro blue tetrazolium chloride), which facilitates the precipitation, to detect alkaline phosphatase activity and alkaline phosphatase labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization.

  • Pink colormetric detection of alkaline phosphatase activity and labels
  • Compatible with a variety of  applications
  • Use alone or in combination with NBT
  • White solid soluble in DMF

For your convenience, we offer a BCIP Pink/NBT kit (10007) that contains both reagents. We also offer Alkaline Phosphatase Conjugated Antibodies.

Find the Right Stain for your Application

The original BCIP forms a dark blue (λmax 615 nm) precipitate and is available in two different salt formulations; BCIP, toluidine salt is soluble in DMF while BCIP, sodium salt is soluble in water. We also offer a Pink BCIP derivative, which produces a pink colored (λmax 540 nm) precipitate. BCIP Red produces a red colored (λmax 565 nm) precipitate. Please see our BCIP Kits that are paired with NBT (nitro blue tetrazolium chloride) for user convenience.

Chemical Structure:

 

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    BCIP Pink/NBT Kit

    NBT and BCIP PINK are widely used together as a chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization. This kit contains 100 mg each of BCIP Pink (10006) and the precipitation inducer NBT (10008).

    BCIP Red/NBT Kit

    A red derivative of BCIP that produces a red (λmax 565 nm) colored precipitate instead of the dark blue precipitate of BCIP. BCIP and derivatives are the most widely used chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications.

    BCIP, Na

    A water soluble formulation of the most widely used chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization.

    BCIP/NBT Kit

    BCIP/NBT are widely used together as a chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization. This kit contains 100 mg each of BCIP sodium salt (10001) and the oxidant NBT (Nitro blue tetrazolium, 10008).

    Chromogenic Phosphatase Substrate Sampler

    The sampler kit contains 10 mg each of BCIP Na (10001), BCIP Pink (10006), BCIP Red (10004) and 250 mg of NBT (10008). NBT forms a co-precipitant and enhances the BCIP reaction.

    Chromogenic β-Galactosidase Substrate Sampler

    For researchers wishing to investigate the suitability of our various chromogenic β-galactosidase substrates, we offer this sampler kit, which contains 5 mg each of the following five different substrates: X-Gal (10011), Red-β-D-Gal (10012), Rose-β-D-Gal (10013), Purple-β-D-Gal (10014) and Green-β-D-Gal (10015).