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A water soluble formulation of the most widely used chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization.

Product Attributes

CAS number


Molecular weight


Storage Conditions

Store at 2 to 8 °C or below, Protect from light, Desiccate


Microscopy, Immunohistology, Western, In situ hybridization, Tissue staining

Molecular Formula: C8H4BrClNNa2O4P

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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100 mg
500 mg
5 g
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Product Description

BCIP (5-Bromo-4-chloro-3-indoxyl phosphate) is the most widely used chromogenic phosphatase substrate, which forms a dark blue (λmax 615 nm) precipitate on enzymatic hydrolysis. It is often used with the oxidant NBT (nitro blue tetrazolium chloride, 10008), which facilitates the precipitation, to detect alkaline phosphatase-activity and -labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization.

  • Colormetric detection of alkaline phosphatase activity and labels
  • Compatible with a variety of  applications
  • Use alone or in combination with NBT
  • White solid soluble in water

For your convenience, we offer a  BCIP/NBT Kit (10003)  that contains both reagents. We also offer Alkaline Phosphatase Conjugated Antibodies.

Find the Right Stain for your Application

The original BCIP forms a dark blue (λmax 615 nm) precipitate and is available in two different salt formulations; BCIP, toluidine salt is soluble in DMF while BCIP, sodium salt is soluble in water. We also offer a Pink BCIP derivative, which produces a pink colored (λmax 540 nm) precipitate. BCIP Red produces a red colored (λmax 565 nm) precipitate. Please see our BCIP Kits that are paired with NBT (nitro blue tetrazolium chloride) for user convenience.

Molecular Structure:


  1. Histochemistry 58, 203 (1978), DOI: 10.1007/bf00495720
  2. Biotechniques 12, 656 (1992), PMID: 1381193
  3. Dev. Dyn., 240, 589 (2011), DOI: 10.1002/dvdy.22544
  4. Dev Comp Immunol. 65, 41 (2016), DOI: 10.1016/j.dci.2016.06.017
  5. Molecular Medicine Reports 15, 1455 (2017), DOI: 10.3892/mmr.2017.6162

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