A water soluble formulation of the most widely used chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization.
BCIP (5-Bromo-4-chloro-3-indoxyl phosphate) is the most widely used chromogenic phosphatase substrate, which forms a dark blue (λmax 615 nm) precipitate on enzymatic hydrolysis. It is often used with the oxidant NBT (nitro blue tetrazolium chloride, 10008), which facilitates the precipitation, to detect alkaline phosphatase-activity and -labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization.
- Colormetric detection of alkaline phosphatase activity and labels
- Compatible with a variety of applications
- Use alone or in combination with NBT
- White solid soluble in water
For your convenience, we offer a BCIP/NBT Kit (10003) that contains both reagents. We also offer Alkaline Phosphatase Conjugated Antibodies.
Find the Right Stain for your Application
The original BCIP forms a dark blue (λmax 615 nm) precipitate and is available in two different salt formulations; BCIP, toluidine salt is soluble in DMF while BCIP, sodium salt is soluble in water. We also offer a Pink BCIP derivative, which produces a pink colored (λmax 540 nm) precipitate. BCIP Red produces a red colored (λmax 565 nm) precipitate. Please see our BCIP Kits that are paired with NBT (nitro blue tetrazolium chloride) for user convenience.
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