There is SDS in the assay buffer of the AccuOrange™ protein concentration kit, but it says the assay can’t tolerate SDS. Why?

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Even though AccuOrange™ buffer does contain SDS, which is required for the dye to bind proteins, the assay is very sensitive to small changes in SDS concentration, and also cannot tolerate non-ionic detergents that form mixed micelles with SDS, like Triton®. Therefore we don’t recommend using the kit for cell lysates or other samples with significant amounts of detergents.

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