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BCIP Red/NBT Kit

A red derivative of BCIP that produces a red (λmax 565 nm) colored precipitate instead of the dark blue precipitate of BCIP. BCIP and derivatives are the most widely used chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications.

Product Attributes

CAS number

298-83-9, 6769-80-8

Applications

Microscopy, Immunohistology, Western, In situ hybridization, Tissue staining

Storage Conditions

Store at 2 to 8 °C or below, Protect from light, Desiccate

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

BCIP Red (5-Bromo-6-chloro-3-indoxyl phosphate, p-toluidine salt) is a derivative of BCIP. While BCIP yields a dark blue precipitating product, BCIP Red produces a red (λmax 565 nm) colored precipitate. BCIP and the derivatives are the most widely used chromogenic phosphatase substrate. They are often used with the oxidant NBT (nitro blue tetrazolium chloride), which facilitates the precipitation, to detect alkaline phosphatase activity and alkaline phosphatase labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization.

  • Red colormetric detection of alkaline phosphatase activity and labels
  • Compatible with a variety of  applications
  • Use BCIP Red alone or in combination with NBT
  • White solid soluble in DMF

This kit contains 100 mg each of BCIP Red (10004) and the oxidant NBT (10008). We also offer Alkaline Phosphatase Conjugated Antibodies.

Find the Right Stain for your Application

The original BCIP forms a dark blue (λmax 615 nm) precipitate and is available in two different salt formulations; BCIP, toluidine salt is soluble in DMF while BCIP, sodium salt is soluble in water. We also offer a Pink BCIP derivative, which produces a pink colored (λmax 540 nm) precipitate. BCIP Red produces a red colored (λmax 565 nm) precipitate. Please see our BCIP Kits that are paired with NBT (nitro blue tetrazolium chloride) for user convenience.

Chemical Structure BCIP Red:

References

  1. Dev Dyn. 236, 1475 (2007)DOI: 10.1002/dvdy.21152
  2. Dev Dyn. 238, 2179 (2009)DOI: 10.1002/dvdy.21883
 

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