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Calcium Calibration Buffer Kit

This kit provides buffers for generating a range of accurate calcium concentrations for the calibration of fluorescent Ca2+ indicators.

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Product Description

This kit provides calibration buffers and a protocol for producing a range of accurate calcium concentrations for the calibration of fluorescent Ca2+ indicators (1,2). Each kit contains 50 mL each of components A and B:

Component A (zero free Ca2+): Zero mM CaEGTA (10 mM K2EGTA,100 mM KCI and 10 mM MOPS, pH 7.20)

Component B (40 uM free Ca2+): 10 mM CaEGTA (10 mM CaEGTA,100 mM KCI and 10 mM MOPS, pH 7.20)

  • Colorless liquids
  • Store at 4°C
  • Preservative-free, use within three months of receipt

References

1) Cell Calcium 12, 279 (1991).

2) Neuropharmacology 34, 1423 (1995).

 

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