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Fluo-3, AM Ester

Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate dye loading.

Product Attributes

CAS number

121714-22-5

Applications

Calcium indicators

Cell permeability

Membrane permeant

Indicator type

Non-ratiometric

Excitation/Emission

506/526 nm (after hydrolysis)

Size
Catalog #
price
Qty
20 x 50 ug
10 x 100 ug
1 mg
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Product Description

Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Fluo-3 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to Fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening. For information on Fluo-3, see catalog number 50010.

Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate dye loading. See the product information sheet for more information.

  • Orange red solid soluble in DMSO
  • Store -20°C and protect from light, especially when in solution
  • C51H50Cl2N2O23
  • MW: 1129.9
  • Abs/Em (after hydrolysis): 506/526 nm (low or high [Ca2 ] )
  • Extinction Coefficient (after hydrolysis): 86,000 M-1 cm-1 (506 nm)
  • Kd (Ca2 ) (after hydrolysis): 450 nM
  • [121714-22-5]

References

1. Cell Calcium 11, 121 (1990).

2. J Cell Biol 111, 183 (1990).

3. J Biol Chem 268, 2134 (1993).

 

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