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D-Luciferin, Free Acid

D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

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CAS number

2591-17-5

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Product Description

D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase. Luciferase is encoded by the luc gene, which is widely used as a reporter gene in a variety of cells. Because of the intrinsic low background of chemiluminescence technique, detection of the luc gene expression can be made at very low level. In addition, luciferin/luciferase has been used to measure 10-15 molar quantity of ATP.

  • White solid soluble in DMSO and slightly soluble in water
  • Store at -20°C and protect from light
  • C11H8N2O3S2
  • Mwt: 280
  • [2591-17-5]

 

References

1. BioTechniques

7, 1116 (1989).

2. Lett. Appl. Microbiol. 1, 208 (1990).

 

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