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D-Luciferin, Potassium Salt

D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

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CAS number

115144-35-9

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Product Description

D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase. Luciferase is encoded by the luc gene, which is widely used as a reporter gene in a variety of cells. Because of the intrinsic low background of chemiluminescence technique, detection of the luc gene expression can be made at very low level. In addition, luciferin/luciferase has been used to measure 10-15 molar quantity of ATP. D-luciferin, potassium salt is a water-soluble form of D-luciferin.

  • Light yellow solid soluble in water
  • Store at -20°C and protect from light
  • C11H7KN2O3S2
  • Mwt: 318.4
  • [115144-35-9]

 

References

1. Appl Opt. 50(10), 1389 (2011).

2. PLoS ONE 5(10), e13545 (2010).

3. Antioxidants and Redox Signaling (2010) doi: 10.1089/ars.2010.3203

4. Biomaterials 31, 1429 (2010).

5. Methods in Molecular Biology (2010) DOI 10.1007/978-1-60761-901-7_11

6. Plant Physiology 139, 1557 (2005).

7. Current Biology 15, 1352 (2005).

8. Cancer Research 64, 6684 (2004).

9. The Plant Cell 16, 769 (2004).

10. PNAS (2003) doi:10.1073/pnas.2435285100

 

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