Support & Resources

+1

Indo-1, AM Ester

Indo-1, AM ester is membrane-permeant and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate cell loading.

Size
Catalog #
price
Qty
10 x 100 ug
1 mg
20 x 50 ug
Clear selection
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Product Description

Indo-1, AM ester is membrane-permeant and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic® F-127 (cat# 59004) is often used as a dispersing agent to facilitate cell loading. Indo-1-AM Ester itself does not bind Ca2+, but it is readily hydrolyzed to indo-1 by endogenous esterases once it enters cells. For more information on indo-1, see cat# 50040.

  • Light yellow solid soluble in DMSO
  • Store desiccated at -20°C and protect from light, especially in solution
  • C47H51N3O22
  • MW: 1010
  • [112926-02-0]


 
 
BAPTA-based ion indicators like Indo-1 have been shown to be fixable in situ by EDC/EDAC (cat# 59002). The fixation of indicator dyes is useful for downstream immunofluorescence and IHC studies (Cell Calcium 1997, 21(3), 175).

As the indicator does not covalently bind to cellular components, it may be actively effluxed from the cell by organic anion transporters. The rate of efflux increases with temperature, and may vary between cell types, resulting in variable retention times of a few minutes to hours. Experiments using indicators in cells usually are performed within one or two hours of loading, but it may be possible to re-load cells with indicator if needed. The organic anion transporter inhibitor Probenecid (#50027) can be used to slow the rate of indicator efflux from cells.
 

Pluronic is a registered trademark of BASF.

References

  1. J Neurophysiol 67, 704 (1992), DOI: 10.1152/jn.1992.67.3.704
  2. J Biol Chem 262, 15041 (1987), PMID: 3667622
  3. J. Biol. Chem. 261, 9710 (1986), PMID: 3755432
  4. Methods Cell Biol, 99, 113, (2021), DOI: 10.1016/B978-0-12-374841-6.00005-0
 

You may also like…

    DMSO, Anhydrous

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

    Pluronic® F-127 20% Solution in DMSO

    Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic dye molecules such as AM esters of calcium indicator dyes to facilitate cell loading.

    Calcium Calibration Buffer Kit

    This kit provides buffers for generating a range of accurate calcium concentrations for the calibration of fluorescent Ca2+ indicators.

    Probenecid, Sodium Salt, Water Soluble

    Probenecid is commonly used to inhibit organic-anion transporters located in the cell membrane. Such transporters can extrude dyes and indicators and thus contribute to low signal or high background in assays due to poor cellular retention of dyes.

    Fluo-4 AM Ester

    Membrane-permeant form of Fluo-4 calcium indicator.

    Fluo-3, AM Ester

    Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate dye loading.