Pluronic® F-127 20% Solution in DMSO
Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic dye molecules such as AM esters of calcium indicator dyes to facilitate cell loading.
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Product Description
Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic dye molecules such as cell-permeable AM esters of Fura-2, Fluo-3, Indo-1, or Rhod-2 calcium indicators to facilitate cell loading.
We also offer Pluronic® F-127 solid and Pluronic® F-127, 10% in H2O.
Pluronic F-127 is a registered trade mark of BASF.
References
1) J. Membrane Biol. 19, 1 (1974).
2) Science 233, 886 (1986).
3) J. Biol Chem 262, 12801 (1987).
4) J. Biol. Chem. 265, 19543 (1990).
5) Methods Enzymol. 302, 341 (1999).
6) Methods Enzymol. 307, 441(1999).
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Fluo-4 AM Ester
Membrane-permeant form of Fluo-4 calcium indicator.
Fluo-3, AM Ester
Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate dye loading.
Rhod-2, AM Ester
Membrane-permeant form of Rhod-2 calcium indicator that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic F-127 is often used as a dispersing agent to facilitate dye loading.
Fura-2, AM Ester
Fura-2, AM ester is a ratiometric calcium indicator that is membrane-permeant and can thus be loaded into cells via incubation.
Indo-1, AM Ester
Indo-1, AM ester is membrane-permeant and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate cell loading.
DMSO, Anhydrous
Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.