Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

NFQ1

NFQ1, a non-fluorescent quencher, is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method employing a novel, non-fluorescent methanethiosulfonate-linked acceptor that can be reversibly coupled to a target sulfhydryl residue via a disulfide bond.

Size
Catalog #
price
Qty
5 mg
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

NFQ1, a non-fluorescent quencher, is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method employing a novel, non-fluorescent methanethiosulfonate-linked acceptor that can be reversibly coupled to a target sulfhydryl residue via a disulfide bond. After the quenched donor emission is quantitated the acceptor is removed by reduction, enabling measurement of unquenched donor emission in the same sample.

  • λAbs: 474 nm
  • Orange solid soluble in DMSO at 20 mM
  • Store desiccated at -20°C and protect from light
  • C17H17N5O6S2
  • MW: 451

References

Biochemistry 2010 doi: 10.1021/bi101188b

 

You may also like…

    DMSO, Anhydrous

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

    TCEP

    TCEP hydrochloride is an odorless reducing agent that is more stable and effective than dithiothreitol (DTT) or 2-Mercaptoethanol. TCEP hydrochloride is soluble in water to 310 grams per liter.

    DTT

    DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.

    My Account

    CF® Dye Maleimides are thiol-reactive fluorescent dyes. Maleimides are commonly used to label proteins on free thiol groups.

    CF® Dye MTS

    MTS CF® Dyes have a fast-reacting and highly selective thiol-reactive methanethiosulfonate (MTS) group, that allows CF® dyes to be conjugated to thiols.

    MTS-BCN

    BCN, an alternative to DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. MTS-BCN is a fast-reacting and highly selective thiol-reactive label that permits one to attach BCN to a thiol group.