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NFQ1

NFQ1, a non-fluorescent quencher, is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method employing a novel, non-fluorescent methanethiosulfonate-linked acceptor that can be reversibly coupled to a target sulfhydryl residue via a disulfide bond.

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Product Description

NFQ1, a non-fluorescent quencher, is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method employing a novel, non-fluorescent methanethiosulfonate-linked acceptor that can be reversibly coupled to a target sulfhydryl residue via a disulfide bond. After the quenched donor emission is quantitated the acceptor is removed by reduction, enabling measurement of unquenched donor emission in the same sample.

  • λAbs: 474 nm
  • Orange solid soluble in DMSO at 20 mM
  • Store desiccated at -20°C and protect from light
  • C17H17N5O6S2
  • MW: 451

References

Biochemistry 2010 doi: 10.1021/bi101188b

 

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