Support & Resources


CF® Dye Maleimides

CF® Dye Maleimides are thiol-reactive fluorescent dyes. Maleimides are commonly used to label proteins, peptides or other molecules containing free thiol groups.

Product Attributes

Chemical reactivity (reacts with)


Functional group


Storage Conditions

Store at -10 to -35 °C, Protect from light

Don’t see what you’re looking for? Contact us for more conjugation options.
Catalog #
0.5 umol
0.25 umol
1 umol
Clear selection
(Please select quantity above )

Wishlist updated! View wishlist

Product Description

CF® Dye Maleimides are thiol-reactive fluorescent dyes. Maleimides are commonly used to label proteins or small ligands like peptides or oligonucleotide thiophosphates on free thiol groups.

  • Thiol-reactive fluorescent dyes.
  • Stably label antibodies, proteins, or small ligands like peptides or oligonucleotide thiophosphates on free thiol groups.
  • Bright, photostable and water-soluble CF® Dyes are excellent options for fluorescent labeling.

Superior CF® Dyes

CF® Dyes are Biotium’s line of next-generation fluorescent dyes that have improved brightness, photostability and water solubility compared to other commercially available fluorescent dyes.

Learn more about CF® Dyes. For more information download the CF® Dye Brochure.

CF® Dye Maleimide

ConjugationEx/EmSizeCatalog No.Dye Features
CF®350355/450 nm1 umol92020CF®350 Features
CF®405S411/431 nm1 umol92030CF®405S Features
CF®405M416/452 nm1 umol92021CF®405M Features
CF®405L413/547 nm1 umol92046CF®405L Features
CF®430424/497 nm1 umol92118CF®430 Features
CF®440433/512 nm1 umol92124CF®440 Features
CF®450448/533 nm1 umol96012CF®450 Features
CF®488A490/516 nm1 umol92022CF®488A Features
CF®503R503/532 nm1 umol96079CF®503R Features
CF®532531/552 nm1 umol92045CF®532 Features
CF®543543/563 nm1 umol92044CF®543 Features
CF®550R551/577 nm1 umol96074CF®550R Features
CF®555554/568 nm1 umol92023CF®555 Features
CF®568562/584 nm1 umol92024CF®568 Features
CF®570568/592 nm1 umol96015CF®570 Features
CF®583584/606 nm1 umol96017CF®583 Features
CF®583R585/609 nm1 umol96107CF®583R Features
CF®594593/615 nm1 umol92025CF®594 Features
CF®620R620/643 nm1 umol92033CF®620R Features
CF®633629/650 nm1 umol92026CF®633 Features
CF®640R642/663 nm1 umol92034CF®640R Features
CF®647652/668 nm1 umol92027CF®647 Features
CF®660R663/682 nm1 umol92031CF®660R Features
CF®660C667/685 nm1 umol92028CF®660C Features
CF®680681/698 nm1 umol92029CF®680 Features
CF®680R680/701 nm1 umol92032CF®680R Features
CF®750755/779 nm0.5 umol96062CF®750 Features
CF®790783/808 nm0.25 umol96108CF®790 Features
CF®800797/817 nm0.25 umol92128CF®800 Features
CF®820822/835 nm0.25 umol96069CF®820 Features


1. J Gen Physiol (2014) 143 (1): 105–118. DOI: 10.1085/jgp.201311053
2. J Biol Chem (2015) 290: 17956-17966. DOI: 10.1074/jbc.M115.641688
3. Nature Methods (2015) 12:773–779. DOI: 10.1038/nmeth.3475
4. Biophys J (2018) 115:996-1006. DOI: 10.1016/j.bpj.2018.07.037

Download a list of CF® Dye references.


You may also like…

    DMSO, Anhydrous

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.


    TCEP hydrochloride is an odorless reducing agent that is more stable and effective than dithiothreitol (DTT) or 2-Mercaptoethanol. TCEP hydrochloride is soluble in water to 310 grams per liter.


    DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.

    CF® Dye MTS

    CF® dye MTS are fast-reacting, highly selective thiol-reactive derivatives for reversible labeling of thiol groups with fluorescent CF® dyes.

    CF® Dye SE/TFP Ester

    CF® Dye SE/TFP esters are amine-reactive fluorescent dyes. They are commonly used to label primary amines of antibodies and other proteins, amine-modified oligonucleotides and other amine-containing molecules.

    CF® Dye Tyramide

    Fluorescent CF® Dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolor immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).