BCN, an alternative to DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. MTS-BCN is a fast-reacting and highly selective thiol-reactive label that permits one to attach BCN to a thiol group.
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CF® Dye Azide
CF® dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.
ViaFluor® SE Cell Proliferation Kits
CF® Dye Maleimides are thiol-reactive fluorescent dyes. Maleimides are commonly used to label proteins on free thiol groups.
CPM (full name: 7-diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin) is probably the most widely used blue fluorescent thiol-reactive dye. This maleimide derivative of coumarin is essentially nonfluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step.
Biotin TS Ethylenediamine
TS biotin ethylenediamine (Biotin ethylenediamine thiosulfate, sodium salt) is a highly selective thiol labeling probe. Similar to the MTS group, TS (thiosulfate) group is capable of reacting with thiols at nearly neutral pH.
Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.
CF® Dye Picolyl Azide
CF® dye picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency. The selected CF® dyes are exceptionally bright and photostable, making them the perfect dye for fluorescence detection.