Support & Resources

+1

Tween® 20

Tween® 20 (polysorbate 20) is a polysorbate surfactant commonly used as a blocking and washing agent in Western blotting and other immunostaining assays. Tween® 20 is typically included in blocking buffers and washing buffers at a concentration of 0.1% to reduce non-specific antibody binding.

Size
Catalog #
price
Qty
50 mL
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Protocols
SDS

Product Description

Tween® 20 (polysorbate 20) is a polysorbate surfactant commonly used as a blocking and washing agent in Western blotting and other immunostaining assays. Tween® 20 is typically included in blocking buffers and washing buffers at a concentration of 0.1% to reduce non-specific antibody binding.

TWEEN is a registered trademark of Uniqema Americas LLC.

References

n/a

 

You may also like…

    Bovine Serum Albumin 30% solution

    Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins such as antibodies and enzymes, and as a blocking agent to reduce non-specific protein binding in immunoassays such as ELISA, immunofluorescence staining, and Western blotting. This is a 30% solution of BSA in water, prepared using IgG- and protease-free BSA.

    10X Fish Gelatin Blocking Agent

    10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.

    Fish Gelatin Powder

    Fish gelatin is commonly used in blocking solutions to minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Because this gelatin is derived from the skin of cold water fish it does not cross-react with mammalian antibodies.

    Dry Milk Powder

    Nonfat dry milk is commonly added to blocking buffers minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Milk can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.

    DAB Substrate Kit

    DAB (3,3'-diaminobenzidine) reacts with HRP in the presence of peroxide to yield an insoluble brown-colored product at locations where peroxidase-conjugated antibodies are bound to samples.

    Peacock™ Prestained Protein Marker

    Peacock™ Prestained Protein Marker is a visible three-color protein ladder with 10 bands ranging from 10 to 180 kDa.