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H2DCFDA

H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein.

Product Attributes

CAS number

4091-99-0

Excitation/Emission

504/529 nm (end product)

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Product Description

H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein.

  • λExEm (pH >6) = 504/529 nm (end product)
  • White solid soluble in DMSO or DMF
  • Store desiccated at -20°C and protect from light
  • C24H16Cl2O7
  • MW: 487.29
  • [4091-99-0]

References

1. Plant Growth Regul (2010) doi: 10.1007/s10725-010-9545-y

2. Physiologia Plantarum (2010) doi: 10.1111/j.1399-3054.2010.01400.x

3. Biochemistry 39, 1040 (2000).

4. Cancer Res. 60, 219 (2000).

5. Free Rad Res 32, 57 (2000).

6. J Immunol Meth 159, 173 (1993).

7. J Immunol Meth159, 131 (1993).

8. Cytometry 13, 615 (1992).

 

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