Support & Resources

+1

5-(and-6)-Carboxy-2′,7′- dichlorofluorescein

5-(and-6)-carboxy-2′,7′-dichlorofluorescein is similar to carboxyfluorescein (51013), but has lower pKa. It is useful for detecting pH in more acidic environment.

Product Attributes

CAS number

111843-78-8

Cell permeability

Membrane impermeant

Probe cellular localization

Fluid phase tracer

pKa

pKa 4.8

Indicator type

Ratiometric

Colors

Green

Excitation/Emission

504/529 nm

Learn more about

Technology

Size
Catalog #
price
Qty
200 mg
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Protocols
SDS

Product Description

5-(and-6)-carboxy-2′,7′-dichlorofluorescein is similar to carboxyfluorescein (51013), but has lower pKa. It is useful for detecting pH in more acidic environment. Also see the membrane-permeant compound dichlorofluorescein diacetate (51016).

  • pKa = 4.8
  • λExλEm = 504/529 nm

  • ε= 90,000
  • Orange solid soluble in DMSO and pH>6 buffers
  • Store at room temperature and protect from light, especially in solution
  • C21H10Cl2O7
  • MW: 445
  • [111843-78-8]

 

References

1. Biochemistry 26, 6330 (1987).

2. Biochim Biophys Acta 985, 75 (1989).

3. Anal Biochem 187, 109 (1990).

 

You may also like…