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5-(and 6)-Carboxy-2′,7′- dichlorofluorescein diacetate

5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate is membrane-permeant and thus can be loaded into cells via incubation. The diacetate is readily hydrolyzed to 5-(and-6)-carboxy-2′,7′-dichlorofluorescein (51015) by intracellular esterases.

Product Attributes

CAS number

127770-45-0

Cell permeability

Membrane permeant

Probe cellular localization

Fluid phase tracer

pKa

pKa 4.8

Indicator type

Ratiometric

Colors

Green

Excitation/Emission

504/529 nm (end product)

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100 mg
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Protocols
SDS

Product Description

5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate is membrane-permeant and thus can be loaded into cells via incubation. The diacetate is readily hydrolyzed to 5-(and-6)-carboxy-2′,7′-dichlorofluorescein (51015) by intracellular esterases.

  • White solid soluble in DMSO
  • Store at 4°C and protect from light, especially in solution
  • C25H14Cl2O9
  • MW: 529
  • [127770-45-0]

References

1. J Cell Biol 111, 877 (1990).

2. Biochemistry 26, 6330 (1987).

3. Biochim Biophys Acta 985,

75 (1989).

4. Anal Biochem 187, 109 (1990).

 

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