Support & Resources

+1

Carboxy-2′,7′- dichlorofluorescein diacetate, succinimidyl ester, 5 and 6 isomers

5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

Product Attributes

pKa

pKa 4.8

Applications

pH indicators

Cell permeability

Membrane permeant

Cellular localization

Nucleus & cytoplasm

Colors

Green

Excitation/Emission

504/529 nm (pH 7) (end product)

Size
Catalog #
price
Qty
25 mg
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Protocols
SDS

Product Description

5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases. The fluorescence of the dye is relatively insensitive to pH change and the labeling has been shown to be stable to formaldehyde or gluteraldehyde fixation.

  • λExEm (of hydrolysis product at pH 7) = 504/529 nm
  • White solid soluble in DMSO or DMF
  • Store at -20°C and protect from light
  • C29H17Cl2NO11
  • MW: 626

References

1. Development 116, 1087 (1992).

 

You may also like…

    CFDA SE, 5 and 6-isomers

    5(6)-CFDA, SE (full name: 5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester, also known as CFSE) is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and non-fluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

    ViaFluor® SE Cell Proliferation Kits

    ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label the cell cytoplasm, and are used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or to track cell populations in mixed co-culture experiments. The labeling withstands fixation and permeabilization for immunostaining.

    Live-or-Dye™ Fixable Viability Staining Kits

    Live-or-Dye™ Fixable Viability Staining Kits stain dead cells during flow cytometry to allow dead cells with permeable plasma membranes to be excluded from analysis.