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Carboxy-2′,7′- dichlorofluorescein diacetate, succinimidyl ester, 5 and 6 isomers

5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

Product Attributes

pKa

pKa 4.8

Cell permeability

Membrane permeant

Probe cellular localization

Nucleus & cytoplasm

Colors

Green

Excitation/Emission

504/529 nm (pH 7) (end product)

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Product Description

5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases. The fluorescence of the dye is relatively insensitive to pH change and the labeling has been shown to be stable to formaldehyde or gluteraldehyde fixation.

  • λExEm (of hydrolysis product at pH 7) = 504/529 nm
  • White solid soluble in DMSO or DMF
  • Store at -20°C and protect from light
  • C29H17Cl2NO11
  • MW: 626

References

1. Development 116, 1087 (1992).

 

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