5-Aminoallyl-dUTP (AA-dUTP), 10 mM solution
5-Aminoallyl-dUTP (AA-dUTP) can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method (1,2).
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5-Aminoallyl-dUTP (AA-dUTP) can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method (1,2). This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled dUTP. AA-dUTP is also available in lyophilized form (catalog no. 40020-1).
- 10 mM solution in TE buffer
- Store at -20°C
- C12H17N3Na3O14P3
- MW: 589.17
References
1. Biol Chem Hoppe Seyler 371, 953 (1990).
2. Biotechniques 28, 518 (2000).