Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

Molecular Biology

Reagents for DNA and Protein Detection, Quantitation & Analysis

Biotium offers many innovative molecular biology solutions. Jump to a section below to learn more:

Safe & sensitive GelRed® and GelGreen® gel stains. DNAzure® Visible DNA gel stain
Fluorescence-based dsDNA quantitation kits with unrivaled sensitivity & linear ranges
EvaGreen® dye, qPCR master mixes, hotstart Taq, polymerase activity kit & more
We've developed dyes that modify dead-cell DNA, allowing selective PCR amplification from live cells
Non-toxic One-Step gel stains, sensitive protein prestains for band quantitation, and other western blot reagents
AccuOrange™ fluorescence protein quantitation assay. GloMelt™ thermal shift stability assay
RNAstorm™ and DNAstorm™ FFPE extraction kits. DNA gel extraction kit
Blue LED light boxes for the visualization of GelGreen® stained gels, or the activation of DNAzure® stained gels
PBS, TBE, ultrapure water, DNA ladders, dNTPs, blocking reagents & more

NUCLEIC ACID GEL STAINS

GelRed® and GelGreen® fluorescent nucleic acid gel stains
The dye experts at Biotium developed the extremely popular GelRed® and GelGreen® fluorescent agarose gel stains to not only be the most sensitive DNA gel stains on the market, but also the safest: they are the only DNA gel stains that have been tested and found to be safe and non-toxic by independent laboratories.

A number of ethidium bromide (EtBr) alternatives are being marketed as being “safe” stains, however many of these dyes are well known to bind DNA in living cells, with cytotoxic effects. GelRed® and GelGreen® are nontoxic and nonmutagenic by virtue of being cell membrane impermeable, so they cannot enter living cells. Download our white paper to learn more about the safety and sensitivity of common DNA gel stains.

  • GelRed® is optimally visualized with UV transilluminators using EtBr detection settings.
  • GelGreen® is optimally visualized with blue light boxes using SYBR® green detection settings.

DNAzure® visible DNA gel stain
DNAzure® is, by far, the most sensitive visible DNA gel stain. This unique gel stain binds to DNA in an agarose gel, and develops visible blue bands after exposure to light. Sensitivity rivals that of fluorescent DNA gel stains.

Gel-Bright™ LED Gel Illuminator
The Gel-Bright™ LED Gel Illuminator was developed by Biotium as a simple and effective device for the visualization of fluorescent green DNA gel stains such as GelGreen®. It is also able to photoactivate the visible blue DNA gel stain, DNAzure®.

Comparison of ethidium bromide (EtBr) and GelRed
Comparison of ethidium bromide (EtBr) and GelRed® in precast gel staining using 1% agarose gel in TBE buffer.
DNA ladder in 1% agarose gel was stained with DNAzure® Blue Nucleic Acid Gel Stain for 25 minutes, and then the visible blue DNA bands were developed for 30 minutes using the Glo-Plate™ Blue LED transilluminator. The gel was placed on a white light transilluminator and imaged with a cell phone camera.

Back to top

Fluorescence-Based dsDNA Quantitation

AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. Some of our kits offer unrivaled sensitivity or linear ranges. To choose the DNA quantitation assay that is right for you, see the linear detection ranges and features table below.

Microplate reader assay features

  • Allow quantitation of many samples and replicates at once, for better accuracy
  • Microplate readers have the highest sensitivity and adjustable excitation and emission settings
  • The AccuBlue® NextGen assay is the most sensitive DNA quantitation assay on the market, allowing accurate detection of 1 pg* of DNA
  • The AccuClear® assay is extremely versatile, with a very broad dynamic range, allowing quantitation of nearly any sample

Qubit® reader assay features

  • AccuGreen™ assay kits designed for use in the pre-programmed dsDNA quantitation assays on the Qubit® reader
  • AccuGreen™ assay kits are direct replacements for the Qubit® assay kits
  • Significant cost savings when compared to the Qubit® kits

Back to top

PCR & DNA Amplification

EvaGreen® dsDNA-binding dye
EvaGreen® dye is a next-generation DNA-binding dye with features ideal for use in qPCR and other applications.  A key feature its novel “release-on-demand” DNA binding mechanism, which permits the use of a relatively high dye concentration in qPCR without PCR inhibition. This makes EvaGreen® dye uniquely suited for both qPCR and High Resolution Melting® (HRM) analysis. EvaGreen® dye is also nonmutagenic, noncytotoxic and safe to aquatic life for direct disposal in the drain.

Forget-Me-Not™ EvaGreen® qPCR master mixes
We offer qPCR master mixes formulated with EvaGreen® dsDNA-binding dye, and master mixes for probe-based qPCR applications.

Our Forget-Me-Not™ EvaGreen® qPCR master mixes are dye-based, hot-start master mixes and can be used in place of any SYBR® Green dye-based master mixes. The Forget-Me-Not™ EvaGreen® qPCR Master Mixes contain a low concentration of inert blue dye allowing you to easily see the wells that contain master mix. The master mixes are available without ROX passive dye, with a separate tube of ROX, or premixed with ROX (high or low concentrations).

Forget-Me-Not™ Universal Probe qPCR master mix
Forget-Me-Not™ Universal Probe qPCR Master Mix is a high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping. This kit is suitable for all fluorescent probe-based technologies, including hydrolysis probes (such as TaqMan® and dual-labeled BHQ® probes) and displacement probes (like Molecular Beacons).

Cheetah™ HotStart Taq
Cheetah™ Taq is a chemically-modified hot-start DNA polymerase. Using a proprietary method developed at Biotium, Cheetah™ Taq is modified to have absolutely no activity at room temperature, but be completely activated after two minutes at 95°C.

EvaEZ™ Fluorometric Polymerase Activity Assay
Our polymerase activity assay kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes. This EvaGreen® dye-based assay can be used to measure the activity of Taq DNA polymerase, Pfu, Vent, Phusion®, Bst, and more.

A comparison of the raw fluorescence signal from qPCR reactions performed with two EvaGreen® master mixes (Forget-Me-Not™ EvaGreen® and Fast EvaGreen®) and QuantiNova SYBR® Green. EvaGreen® dye is less inhibitory than SYBR® green, allowing for a much brighter signal.

 

Sample data from an EvaEZ assays, showing a titration of Taq DNA Polymerase.

We also sell a variety of PCR accessory reagents & related products, such as dNTPs, fluorescently-labeled and biotinylated nucleotides (dUTP, dCTP & others), ROX Reference Dye, and HotStart and WarmStart™ Polymerase Modification Kits.

Back to top

VIABILITY PCR DYES & KITS

Viability PCR (v-PCR) is a powerful technology for the sensitive and rapid detection of viable microorganisms. qPCR detection is faster and more sensitive than culturing methods, but it does not distinguish between live and dead cells. On the other hand, v-PCR using PMAxx™ or PMA viability dyes can discriminate between live and dead organisms. V-PCR technology can be applied not only to bacteria but to other organisms like yeast and viruses.

How does v-PCR work?
PMAxx™ and PMA are photoreactive dyes with high affinity for DNA. These dyes are membrane-impermeable, but can enter dead cells with compromised membranes. The dyes intercalate into dsDNA and form a covalent linkage upon exposure to intense visible light. The reaction inhibits PCR amplification of modified DNA templates by a combination of removal of modified DNA during purification and inhibition of template amplification by DNA polymerases.

We also offer PMA-Lite™ & Glo-Plate™ Blue LED photolysis devices for light treatment samples in tubes or plates.

Live or dead bacteria treated with either PMA or PMAxx™ prior to qPCR. Dead bacteria treated with either PMA or PMAxx™ show delays in amplification compared to either live cells, or untreated dead cells. Bacteria treated with PMAxx™ show a larger delay than those treated with PMA. Therefore PMAxx™ is better able to differentiate live vs dead bacteria by viability PCR.

Back to top

PROTEIN GEL STAINS

One-Step protein gel stains
Our One-Step protein gel stains are low-cost, quick, and easy-to-use. Simply add stain to your SDS-PAGE gel without fixing or washing, and stain for 5 to 60 min. These stains are non-toxic for safer handling, and are certified under CCR Title 22 as non-hazardous for drain disposal after pH neutralization.

  • One-Step Blue® replaces Coomassie® for visible staining or near-IR fluorescence.
  • One-Step Lumitein replaces SYPRO® Ruby for UV box or gel scanner.
  • One-Step Lumitein™ UV replaces Oriole™ for UV transilluminator.

Mix-n-Stain™ Total Protein Prestain Kits
These prestains allow simple and highly sensitive protein quantitation on SDS-PAGE gels and western blot membranes. The kits allow you to label purified proteins or cell lysates with our near-infrared CF® dyes before running the samples on SDS-PAGE. The staining demonstrates excellent linearity for quantitation of total protein over a wide dynamic range, outperforming traditional western blot normalization based on housekeeping protein detection.

Visible One-Step Blue® staining (left), fluorescent One-Step Lumitein™ staining that can be imaged on a UV box or fluorescence gel scanner (right).

Conjugates and accessories for western blotting
We offer wide selection of primary and secondary antibodies conjugated to our exceptional near-IR CF® dyes for western blot, as well as HRP conjugates for chemiluminescence detection. We also offer a selection of protein markers, buffers, blocking agents, and more.

Back to top

Protein Quantitation & Analysis

GloMelt™ Thermal Shift Protein Stability Kits
Our GloMelt™ dye undergoes fluorescence enhancement upon binding to hydrophobic regions of denatured proteins. It can be used for measuring a protein’s denaturation temperature in an assay called thermal shift assay (also called differential scanning fluorimetry or Thermofluor). This assay can be used to screen conditions that affect protein thermal stability, such as mutations, ligand binding, and buffer formulations. The assay is rapid and is performed on a quantitative PCR system. The GloMelt™ dye has advantages over competitor products, such strong signal, and compatibility with detergents and reducing agents.

 

 

AccuOrange™ Protein Quantitation Kit
This is a highly sensitive fluorescence-based assay for quantitating purified protein or antibody samples in 96-well format. The detection range of the assay is 0.1-15 ug/mL protein. AccuOrange™ is much more sensitive than traditional protein quantitation assays such as BCA, Bradford and Lowry, and is more linearity and reproducible compared to the NanoOrange® protein quantitation assay. The assay shows minimal variability between different proteins, and has stable fluorescence signal for up to 16 hours. The AccuOrange™ assay has low tolerance for detergents, and is not recommended for use with cell lysates.

GloMelt™ dye can be used to monitor the temperature dependent unfolding of a protein.
The AccuOrange™ fluorescent protein quantitation assay is more  linear and less variable than the NanoOrange® assay.

Back to top

DNA & RNA extraction kits

RNAstorm™ and DNAstorm™ FFPE extraction kits
Our proprietary catalytic CAT5™ technology greatly accelerates the removal of formaldehyde damage and allows recovery of higher quality nucleic acids from FFPE samples. This results in higher yields, more intact RNA or DNA, and better results in downstream analysis like PCR, microarray, or next-generation sequencing (NGS).

CAT5™ catalytic technology

  • Enzymatic reversal of formaldehyde crosslinking
  • Effective: Higher yields than heat-based methods
  • Gentle: no high temperature or harsh solvents
  • Higher quality RNA or DNA with less fragmentation
rna and dna extraction kits comparison
RNA extracted using the RNAstorm™ kit was compared to a popular commercial kit. Increases in DV200 (the percentage of RNA with length > 200 nt) were seen for a variety of tissue samples.

DNA Gel Extraction Kit
Our DNA Gel Extraction Kit is a silica-gel spin column based DNA extraction kit designed to purify 40 bp-40 kb DNA fragments from agarose gels in TAE or TBE buffer. Each column has a maximum binding capacity of 10 ug DNA. Biotium’s DNA Gel Extraction Kit removes GelRed™ and GelGreen™ Nucleic Acid Gel Stains, along with other commonly used nucleic acid gel stains. The purified DNA is compatible with common downstream applications such as transformation, sequencing, PCR, and restriction digests.

Back to top

LED GEL ILLUMINATORS


Gel-Bright™ LED Gel Illuminator
The Gel-Bright™ LED Gel Illuminator was developed by Biotium as a simple, effective device for the visualization of fluorescent green DNA gel stains such as GelGreen®. It is also able to photoactivate the visible blue DNA gel stain, DNAzure®.

Features:

  • Optimized for green dyes, such as GelGreen® DNA Gel Stain
  • Safer than a UV transilluminator; UV protective eyewear or clothing are not  required
  • No UV-induced damage of DNA
Gel-Bright™ LED Gel Illuminator

Glo-Plate™ Blue LED Illuminator
The Glo-Plate™ Blue LED Gel Illuminator  was designed to photoactivate PMA & PMAxx™ viability PCR dye-treated samples in a 96-well plate format.  It can also be used to activate the visible blue DNA gel stain, DNAzure®.

Features:

  • Blue LEDs arranged under a diffuser plate allow even illumination from the bottom
  • 4 timer settings
  • Simple, lightweight design with a small footprint makes it easily portable
Glo-Plate™ Blue LED Illuminator

Back to top

Buffers & Accessories


We sell a variety of PCR accessory reagents & related products, such as DNA ladders, dNTPs, fluorescently-labeled and biotinylated nucleotides (dUTP, dCTP & others), ROX Reference Dye. See our product listing here.

We also sell many common buffers and accessory products such as PBS, TBE, blocking reagents (like fish gelatin, dry milk powder, BSA), activated charcoal decontamination bags, RNase-Free calf thymus DNA, and much more. See our products here.

Back to top