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dUTP CF® Dye Conjugates

Labeled with our superior CF® dyes, dUTP conjugates can be used for TUNEL assay or to synthesize labeled DNA probes for in situ hybridization and nucleic acid blotting applications.

Product Attributes


Blue, Green, Orange, Red, Far-red, Near-infrared

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Catalog #
5 nmol
25 nmol
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Product Description

CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labeled DNA probes for in situ hybridization and nucleic acid blotting applications.

  • Synthesize exceptionally bright and photostable fluorescent DNA probes
  • Detect by fluorescence microscopy, flow cytometry, or in gel
  • Compatible with fixed cells or tissue sections
  • Choice of 8 CF® dye colors from blue to near infrared
  • Biotium’s CF® dyes have superior brightness, photostability, and are water-soluble

Supplied in lyophilized form. For PCR applications, Taq polymerase should be used with dUTP conjugates, because dUTP inhibits archaeal polymerases such as Pfu and Vent®. Also see our CF® Dye dCTP Conjugates and TUNEL assay kits, available with a wide selection of bright and photostable CF® dyes.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.

dUTP CF® Dye Conjugates

Product Dye Ex/Em Size SKU Purchase
CF®405S dUTP1 CF®405S 404/431 nm 5 nmol 40004-T Purchase 40004-T
25 nmol 40004 Purchase 40004
CF®405M dUTP CF®405M 408/452 nm 5 nmol 40100-T Purchase 40100-T
25 nmol 40100 Purchase 40100
CF®488A dUTP CF®488A 490/515 nm 5 nmol 40008-T Purchase 40008-T
25 nmol 40008 Purchase 40008
CF®543 dUTP CF®543 541/560 nm 5 nmol 40002-T Purchase 40002-T
25 nmol 40002 Purchase 40002
CF®568 dUTP CF®568 562/583 nm 5 nmol 40005-T Purchase 40005-T
25 nmol 40005 Purchase 40005
CF®594 dUTP CF®594 593/614 nm 5 nmol 40006-T Purchase 40006-T
25 nmol 40006 Purchase 40006
CF®640R dUTP CF®640R 642/662 nm 5 nmol 40007-T Purchase 40007-T
25 nmol 40007 Purchase 40007
CF®680R dUTP2 CF®680R 680/701 nm 5 nmol 40003-T Purchase 40003-T
25 nmol 40003 Purchase 40003
1. CF®405S-dUTP may not be suitable for TUNEL staining in tissues due to blue autofluorescence in tissues and lower incorporation efficiency in tissue sections compared to other CF®dye dUTP conjugates. 2. CF®680R-dUTP has been tested in TUNEL staining of cells, but may not be efficiently incorporated in tissue sections.


CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific. Vent is a registered trademark of New England Biolabs, Inc.


Download a list of CF® dye references.

  1. Nat Commun, 10, (2019), 967; DOI: 10/gfv3m9
  2. Molecular Cell, 75, (2019), 267; DOI: 10/ggvb22
  3. bioRxiv, (2019), 536136; DOI: 10/ggvb2w
  4. Nat Commun, 7, (2016), 10966; DOI: 10/f8dxfd
  5. Nucleus, 7, (2016), 325; DOI: 10/ggvb24
  6. Cell Death Differ, 19, (2012), 245; DOI: 10/btnptc

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