dUTP CF® Dye Conjugates
CF® dye-dUTP can be used for TUNEL assay, or to synthesize labeled DNA probes for in situ hybridization and nucleic acid blotting applications.
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5-Aminoallyl-dUTP (AA-dUTP), 10 mM solution
5-Aminoallyl-dUTP (AA-dUTP) can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method (1,2).
Cyanine Dye dUTP
Cyanine 555-dUTP and Cyanine 647-dUTP can be used to synthesize labeled DNA probes for in-situ hybridization, microarray or blotting techniques. Cyanine 555 is structurally identical to Cy®3 while Cyanine 647 is structurally identical to Cy®5 from GE Healthcare.
TUNEL Assay Apoptosis Detection Kit
The CF® dye TUNEL Assay Apoptosis Detection Kit employs dUTP conjugated to the exceptionally bright and photostable fluorescent dyes, for single step fluorescent TUNEL labeling of fixed cells or tissues for analysis by fluorescence microscopy or flow cytometry.
dCTP CF® Dye Conjugates
CF® dye dCTP can be used to synthesize labeled DNA probes for in-situ hybridization, microarray or blotting applications.
Digoxigenin-dUTP, Alkali Stable
Digoxigenin-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3'-end terminal labeling to synthesize labeled DNA probes for in-situ hybridization, microarray or blotting techniques. The digoxigenin labeled probe could be detected by using fluorescent labeled or enzyme labeled anti-digoxigenin antibody.