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TUNEL Assay Apoptosis Detection Kit

The CF® dye TUNEL Assay Apoptosis Detection Kit employs dUTP conjugated to the exceptionally bright and photostable fluorescent dyes, for single step fluorescent TUNEL labeling of fixed cells or tissues for analysis by fluorescence microscopy or flow cytometry.

Product Attributes

Apoptosis/viability marker

DNA fragmentation

For live or fixed cells

For fixed cells/tissue sections

Detection method/readout

Fluorescence microscopy, Flow cytometry

Assay type/options

Endpoint assay

Fixation options

Permeabilize before staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Colors

Green, Red, Far-red

Conjugation
Size
Catalog #
price
Qty
50 reactions
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Product Description

In the TUNEL assay, TdT enzyme catalyzes the addition of labeled dUTP to the 3′- ends of the internucleosomal DNA strand breaks that are a hallmark of apoptosis. The CF® dye TUNEL Assay Apoptosis Detection Kit employs dUTP conjugated to the exceptionally bright and photostable fluorescent dyes, for single step fluorescent TUNEL labeling of fixed cells or tissues for analysis by fluorescence microscopy or flow cytometry.

The kit is available with the following CF® dyes: CF®488A, CF®594, and CF®640R.

References

1. Parrish and Xue (2006). Chromosoma 115: 89-97
2. Gold et al. (1994). Lab Invest. 71(2):219-25.
3. PLoS ONE 8(1), e53657 (2013).
4. Cell Death and Differentiation (2013), http://dx.doi.org/10.1038/cdd.2013.152
5. Kapoor N, et al. (2013) Human Granuloma In Vitro Model, for TB Dormancy and Resuscitation.PLoS ONE 8(1): e53657. doi:10.1371/journal.pone.0053657

Download a list of CF® dye references.

Cat#30063
1. PLoS ONE (2017), https:// doi.org/10.1371/journal.pone.0187270

 

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