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5X Passive Lysis Buffer

5X Passive Lysis Buffer is concentrated lysis buffer designed for use with Biotium’s Renilla Luciferase Assay Kit (30004) or Firefly and Renilla Dual Luciferase Assay Kit (30005).

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Product Description

5X Passive Lysis Buffer is concentrated lysis buffer designed for use with Biotium’s Renilla Luciferase Assay Kit 2.0 (catalog no. 30082). Those kits are supplied with sufficient 5X Passive Lysis Buffer to complete the stated number of assays using 96-well to 24-well plates. 5X Passive Lysis Buffer is sold separately for use with the kits when more lysis buffer (e.g., >100 uL per well) is required.

More Luciferase Kits

Catalog numberProductDescription
30085Firefly Luciferase Assay Kit 2.0Flash-type firefly luciferase assay kit.
30075Firefly Luciferase Assay Kit (Lyophilized)Firefly luciferase assay kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C.
999235X Firefly Lysis BufferExtra lysis buffer for use with Firefly Luciferase Assay Kits, only required if using well sizes larger than 24-well.
30028Steady-Luc™ Firefly HTS Assay KitGlow-type firefly luciferase assay with signal half-life of about 3 hours for high-throughput screening in multi-well plates.
30028LSteady-Luc Firefly HTS Assay Kit (Lyophilized)Steady-Luc kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C.
30082Renilla Luciferase Assay Kit 2.0Flash-type Renilla luciferase assay kit.
999125X Passive Lysis BufferExtra lysis buffer for use with Renilla Luciferase Assay Kits, only required if using well sizes larger than 24-well.
30081Firefly and Renilla Luciferase Single Tube Assay KitAssay kit for measuring firefly and Renilla activities sequentially in the same sample in a single tube.
99821-100mL1X Passive Lysis Buffer 2.0 Extra lysis buffer for use with Firefly & Renilla Single Tube Assay Kits, only required if using well sizes larger than 24-well.
30020ATP-Glo™ Bioluminometric Cell Viability Assay KitFlash-type luminescence-based assay for measuring cell viability or ATP concentration.
22003Mini Cell ScrapersMini-cell scrapers, useful for lysing cells in 96-well, 48-well, or 24-well plates.

References

  1. Bulletin Chem Soc. J (2016), doi:10.1246/bcsj.20150405
  2. PLoS ONE (2013), doi:10.1371/journal.pone.0053936
  3. Biol Syst (2012), https://www.omicsonline.org/open-access/targeting-of-calcitriol-to-inflammatory-breast-cancer-tumors-and-metastasis-in-vitro-and-in-vivo-BSO.1000104.php?aid=10793
  4. Biol Syst (2015), http://dx.doi.org/10.4172/2329-6577.1000104
 

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