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5X Passive Lysis Buffer

Additional concentrated lysis buffer for use with our Renilla Luciferase Assay Kits in large volume assays.

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Storage Conditions

Store at -10 to -35 °C or below

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30 mL
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Product Description

5X Passive Lysis Buffer is concentrated lysis buffer designed for use with our Renilla Luciferase Assays when additional volumes of lysis buffer are required.

  • Additional 5X Passive Lysis Buffer for larger volume assays
  • For use with Renilla Luciferase Assay Kit 2.0 (30082)

Our kits are supplied with sufficient 5X Passive Lysis Buffer to complete the stated number of assays using 96-well to 24-well plates. 5X Passive Lysis Buffer is sold separately for use with the kits when more lysis buffer (e.g., >100 uL per well) is required. If you require additional lysis buffer for use with Firefly & Renilla Luciferase Single Tube Assay Kit (30081),  use 1X Passive Lysis Buffer 2.0 (99821-100mL). If you require additional lysis buffer for use with Firefly Luciferase Assay Kit 2.0 (30085) or Firefly Luciferase Assay Kit (Lyophilized) (30075), use 5X Firefly Luciferase Lysis Buffer (99923).

More Luciferase Kits

Catalog number Product Description
30085 Firefly Luciferase Assay Kit 2.0 Flash-type firefly luciferase assay kit.
30075 Firefly Luciferase Assay Kit (Lyophilized) Firefly luciferase assay kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C.
99923 5X Firefly Lysis Buffer Extra lysis buffer for use with Firefly Luciferase Assay Kits, only required if using well sizes larger than 24-well.
30028 Steady-Luc™ Firefly HTS Assay Kit Glow-type firefly luciferase assay with signal half-life of about 3 hours for high-throughput screening in multi-well plates.
30028L Steady-Luc Firefly HTS Assay Kit (Lyophilized) Steady-Luc kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C.
30082 Renilla Luciferase Assay Kit 2.0 Flash-type Renilla luciferase assay kit.
99912 5X Passive Lysis Buffer Extra lysis buffer for use with Renilla Luciferase Assay Kits, only required if using well sizes larger than 24-well.
30081 Firefly and Renilla Luciferase Single Tube Assay Kit Assay kit for measuring firefly and Renilla activities sequentially in the same sample in a single tube.
99821-100mL 1X Passive Lysis Buffer 2.0 Extra lysis buffer for use with Firefly & Renilla Single Tube Assay Kits, only required if using well sizes larger than 24-well.
30020 ATP-Glo™ Bioluminometric Cell Viability Assay Kit Flash-type luminescence-based assay for measuring cell viability or ATP concentration.
22003 Mini Cell Scrapers Mini-cell scrapers, useful for lysing cells in 96-well, 48-well, or 24-well plates.

References

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  6. Front. Immunol., 9,1564, (2018), DOI: 10.3389/fimmu.2018.01564
  7. J. Orthop. Res., 35, 876, (2017), DOI: 10.1002/jor.23342
  8. Bulletin Chem Soc. J (2016), DOI: 10.1246/bcsj.20150405
  9. PLoS ONE, 8(1), e53936 (2013), DOI: 10.1371/journal.pone.0053936
  10. Biol Syst 1, 1, (2012), DOI: 10.4172/bso.1000104
  11. Int J Biochem Mol Biol, 2(4), 318, (2011), PMCID: PMC3242428
 

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