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Activated charcoal in a convenient bag for decontamination of used gel staining solutions containing ethidium bromide or other toxic dyes.
The Activated Charcoal Decontamination Bags were developed for convenient, no-mess clean-up of mutagenic dyes like ethidium bromide (EtBr) from used gel staining solutions.
The Activated Charcoal Decontamination Bags concentrate ethidium bromide from used gel staining solutions in a convenient bag. Concentrating the toxic dye into a small bag allows the user to dispose of it in the solid waste instead of as a large amount of liquid waste, potentially saving a lot of money in toxic waste disposal fees.
Each bag has a total capacity of at least 10 mg ethidium bromide. The bags can also be used to decontaminate other solutions containing mutagenic or toxic dyes. The Biotium gel stains GelRed® and GelGreen® have been independently certified as non-mutagenic and non-toxic, and can therefore be disposed of directly down the drain. However, since local waste procedures may vary, the Activated Charcoal Decontamination Bags can be used to remove GelRed® and GelGreen®.
The bags come with instructions for decontaminating buffers and monitoring dye removal.
We and other users have often observed that GelGreen® stains ssDNA and RNA orange/ pink and dsDNA green. We have also seen that smaller dsDNA fragments can appear orange-pink, the color ranging from white-pink-orange. We are not sure about the underlying mechanism, possibly the structure of single-stranded nucleic acids favors an altered stacking interaction of GelGreen® monomers leading to the formation of J-aggregates that have red emission.
Yes, use the post-staining protocol for polyacrylamide gels. For polyacrylamide gels containing 3.5-10% acrylamide, typical staining time is 30 minutes to 1 hour with gels of higher acrylamide content requiring longer staining time.,
Biotium also offers PAGE GelRed® a non-toxic, non-mutagenic dye specifically designed for staining DNA in polyacrylamide gels.
We don’t recommend adding GelRed® or GelGreen® directly to loading buffer, because this can result in inaccurate band migration. Biotium offers 6X GelRed® Prestain Loading Buffers designed for this application, although we do not recommended them for applications where precise DNA band sizing is required. For the most accurate determination of DNA band sizes, we recommend using GelRed® post-staining (see the GelRed® protocol for details).