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PAGE GelRed® Nucleic Acid Gel Stain

PAGE GelRed® is a non-toxic, non-mutagenic red DNA gel stain specifically designed to stain DNA in polyacrylamide gels. PAGE GelRed® can be imaged using a 254 nm UV transilluminator with an ethidium bromide filter.

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10,000X in water, 1X in water

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0.1 mL
500 uL
4 L
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Product Description

PAGE GelRed® is a non-toxic, non-mutagenic red DNA gel stain specifically designed to stain DNA in polyacrylamide gels. PAGE GelRed® can be imaged using a 254 nm UV transilluminator with an ethidium bromide filter. While PAGE GelRed® also can be used to stain DNA in agarose gels, Biotium’s original GelRed® nucleic acid gel stain (catalog number 41003) is more sensitive for agarose gel applications. PAGE GelRed® can be removed from DNA after agarose gel staining using commonly available gel extraction kits. PAGE GelRed® is 6-8 times more sensitive for dsDNA over RNA.

PAGE GelRed® was subjected to a series of tests at Biotium and two independent laboratories to assess the dye’s safety for routine handling and disposal. Laboratory tests show that the dye is impenetrable to latex or nitrile gloves and cell membranes. Unlike the highly mutagenic EtBr and the reportedly mutation-enhancing SYBR® Green I (1), PAGE GelRed® is non-toxic and non-mutagenic in bacterial AMES tests at concentrations well above the working concentrations used in gel staining. PAGE GelRed® successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization, under which it is classified as non-hazardous waste. A complete safety report is available under the downloads tab.

PAGE GelRed® is available as a ready-to-use staining solution at 1X in water, or a concentrated dye solution at 10,000X in water.

Product / MethodProcedureAdvantagesDisadvantagesRecommended for
DNA prestaining with GelRed® Prestain Plus 6X DNA Loading DyeGelRed® loading buffer is added directly to the DNA sample before loading• Fast & simple: one-step sample loading & DNA staining

• Less concentrated dye for safer handling

• Can re-run a gel to use empty lanes
• Not recommended for PAGE, DGGE, EMSA, or PFGE gels

• Dye may cause band migration issues when loading larger amounts of DNA (more than ~100 ng/band), or for some restriction digests
• Routine agarose gels

• Recommended loading 50-200 ng ladder or 2-5 uL PCR product ( ~100 ng/band or less)
Precast gel staining with GelRed® 10,000X in waterGelRed® is mixed with molten agarose before gel castingFamiliar protocol, rapid results
Precast gel staining with GelRed® Agarose LE
Agarose is supplied pre-coated with GelRed®, just dissolve, heat, and pourSafer & more convenient, no need to handle concentrated dye
Post-electrophoresis gel staining with GelRed® 10,000X in water
- or -
GelRed® 3X in water
No fluorescent dye is added to the gel, it is stained in 3X GelRed® solution after electrophoresis• Most accurate sizing/sharpest bands

• Staining solution can be re-used

• Enhance sensitivity by adding NaCl
Extra staining step (up to 30 minutes) after electrophoresis (some customers report good results after only 5 minutes if dye is not reused)• Highly accurate band sizing

• Gels with more than ~100 ng DNA per band

• Analyzing restriction digests
Post-electrophoresis gel staining of PAGE gels using PAGE GelRed® 10,000X or 1X in waterNo fluorescent dye is added to the gel, it is stained in 1X PAGE GelRed® solution after electrophoresis• Formulated for efficient penetration and staining of polyacrylamide gels

• Like the classic GelRed®, it is safe and environmentally friendly
Extra staining step of approx. 30 minutes after electrophoresisStaining of nucleic acids in PAGE gels

References

1. Mutation Research 492, 91 (2001).

 

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