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PAGE GelRed Nucleic Acid Gel Stain

PAGE GelRed™ is a non-toxic, non-mutagenic red DNA gel stain specifically designed to stain DNA in polyacrylamide gels. PAGE GelRed™ can be imaged using a 254 nm UV transilluminator with an ethidium bromide filter.

100 bp DNA Ladder in TE Buffer
Catalog #

41014, 41008-500uL, - 41008-TView allHide

Size

0.1 mL, 500 uL, 4 L

Format

10,000X in water, 1X in water

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0.1 mL
500 uL
4 L
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Product Description

PAGE GelRed™ is a non-toxic, non-mutagenic red DNA gel stain specifically designed to stain DNA in polyacrylamide gels. PAGE GelRed™ can be imaged using a 254 nm UV transilluminator with an ethidium bromide filter. While PAGE GelRed™ also can be used to stain DNA in agarose gels, Biotium’s original GelRed™ nucleic acid gel stain (catalog number 41003) is more sensitive for agarose gel applications. PAGE GelRed™ can be removed from DNA after agarose gel staining using commonly available gel extraction kits. PAGE GelRed™ is 6-8 times more sensitive for dsDNA over RNA.

PAGE GelRed™ was subjected to a series of tests at Biotium and two independent laboratories to assess the dye’s safety for routine handling and disposal. Laboratory tests show that the dye is impenetrable to latex or nitrile gloves and cell membranes. Unlike the highly mutagenic EtBr and the reportedly mutation-enhancing SYBR® Green I (1), PAGE GelRed™ is non-toxic and non-mutagenic in bacterial AMES tests at concentrations well above the working concentrations used in gel staining. PAGE GelRed™ successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization, under which it is classified as non-hazardous waste. A complete safety report is available under the downloads tab.

PAGE GelRed™ 1X in water is a ready-to use staining solution that is available in 1X in water and 10,000X in water.

References

1. Mutation Research 492, 91 (2001).

 

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Support & Faq

GelRed™ and GelGreen™ Nucleic Acid Gel Stains

The main difference between GelRed™ and GelGreen™ is their fluorescence excitation and emission wavelengths. GelRed™ has red fluorescence, similar to ethidium bromide. GelGreen™ has green fluorescence, similar to SYBR® Green or SYBR® Safe. Both dyes are compatible with standard UV transilluminators. GelGreen™ is also compatible with blue light transilluminators, which allow users to avoid exposing themselves and their DNA samples to ultraviolet radiation.

GelRed™ and GelGreen™ have higher sensitivity for double stranded nucleic acids compared to single stranded nucleic acids, but GelRed™ is more sensitive for staining single stranded nucleic acids than GelGreen™. GelRed™ is about twice as sensitive for double stranded nucleic acids compared to single-stranded nucleic acids, and about five times more sensitive than GelGreen™ for staining single stranded nucleic acids.

For more information about these products, please visit our DNA stains technology page.

View the GelRed™ Product Line

View the GelGreen™ Product Line

Category: GelRed™ and GelGreen™ Nucleic Acid Gel Stains

← FAQs

The water formulation is a newer and improved product compared to the stock in DMSO. We recommend using dyes in water to avoid the potential hazards of handling DMSO, which can be absorbed through the skin. We continue to offer dyes in DMSO because some users do not wish to alter their established laboratory protocols. Based on internal testing, both formulations perform similarly.

Category: GelRed™ and GelGreen™ Nucleic Acid Gel Stains

← FAQs

GelRed™ is compatible with a standard UV transilluminator (302 or 312 nm).
GelGreen™ has sufficient absorption between 250-300 nm and a strong absorption peak at around 500 nm. GelGreen™ is compatible with a 254 nm UV transilluminator or a gel reader with visible light excitation such as a Dark Reader or a 488 nm laser gel scanner.

Category: GelRed™ and GelGreen™ Nucleic Acid Gel Stains

← FAQs

← FAQs

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.