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PAGE GelGreen® Nucleic Acid Gel Stain

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10,000X in water, 1X in water

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Product Description

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PAGE GelGreen® is a non-toxic, non-mutagenic green DNA gel stain specifically designed to stain DNA in polyacrylamide gels. PAGE GelGreen® can be imaged using a 254 nm UV transilluminator with a SYBR® Green filter, or with gel readers equipped with visible light excitation, such as Dark Reader® imaging systems or 488 nm laser-based gel scanners. While PAGE GelGreen® also can be used to stain DNA in agarose gels, Biotium’s original GelGreen® nucleic acid gel stain (catalog number 41005) is more sensitive for agarose gel applications. PAGE GelGreen® can be removed from DNA after agarose gel staining using commonly available gel extraction kits. PAGE GelGreen® is 2-3 times more sensitive for dsDNA over RNA. For detailed information, please visit the DNA Stain Technology page.

PAGE GelGreen® was subjected to a series of tests at Biotium and two independent laboratories to assess the dye’s safety for routine handling and disposal. Laboratory tests show that the dye is impenetrable to latex or nitrile gloves and cell membranes. Unlike the highly mutagenic EtBr and the reportedly mutation-enhancing SYBR® Green I (1), PAGE GelGreen® is non-toxic and non-mutagenic in bacterial AMES tests at concentrations well above the working concentrations used in gel staining. PAGE GelGreen® successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization, under which it is classified as non-hazardous waste. A complete safety report is available under the downloads tab.

PAGE GelGreen® is a ready-to-use staining solution that is available in 1X in water and 10,000X in water. Also see PAGE GelRed®.

SYBR is a registered trademark of Thermo Fisher Scientific. Dark Reader is a registered trademark of Clare Chemical.


1. Mutation Research 492, 91 (2001).


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Support & Faq

GelRed® and GelGreen® Nucleic Acid Gel Stains

The main difference between GelRed® and GelGreen® is their fluorescence excitation and emission wavelengths. GelRed® has red fluorescence, similar to ethidium bromide. GelGreen® has green fluorescence, similar to SYBR® Green or SYBR® Safe. Both dyes are compatible with standard UV transilluminators. GelGreen® is also compatible with blue light transilluminators, which allow users to avoid exposing themselves and their DNA samples to ultraviolet radiation.

GelRed® and GelGreen® have higher sensitivity for double stranded nucleic acids compared to single stranded nucleic acids, but GelRed® is more sensitive for staining single stranded nucleic acids than GelGreen®. GelRed® is about twice as sensitive for double stranded nucleic acids compared to single-stranded nucleic acids, and about five times more sensitive than GelGreen® for staining single stranded nucleic acids.

For more information about these products, please visit our DNA stains technology page.

View the GelRed® Product Line

View the GelGreen® Product Line

Category: GelRed® and GelGreen® Nucleic Acid Gel Stains

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The water formulation is a newer and improved product compared to the stock in DMSO. We recommend using dyes in water to avoid the potential hazards of handling DMSO, which can be absorbed through the skin. We continue to offer dyes in DMSO because some users do not wish to alter their established laboratory protocols. Based on internal testing, both formulations perform similarly.

Category: GelRed® and GelGreen® Nucleic Acid Gel Stains

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GelRed® is compatible with a standard UV transilluminator (302 or 312 nm).
GelGreen® has sufficient absorption between 250-300 nm and a strong absorption peak at around 500 nm. GelGreen® is compatible with a 254 nm UV transilluminator or a gel reader with visible light excitation such as a Dark Reader or a 488 nm laser gel scanner.

Category: GelRed® and GelGreen® Nucleic Acid Gel Stains

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