PAGE GelGreen® Nucleic Acid Gel Stain

This product is currently unavailable and out of stock.

This product is available by special order only.

Please fill in the inquiry form and we will contact you shortly.

Don't see what you're looking for? Contact us for more conjugation options.

GelRed and GelGreen safe, bright nucleic acid gel stains

Product Description

This product is currently unavailable and out of stock.

PAGE GelGreen® is a non-toxic, non-mutagenic green DNA gel stain specifically designed to stain DNA in polyacrylamide gels. PAGE GelGreen® can be imaged using a 254 nm UV transilluminator with a SYBR® Green filter, or with gel readers equipped with visible light excitation, such as Dark Reader® imaging systems or 488 nm laser-based gel scanners. While PAGE GelGreen® also can be used to stain DNA in agarose gels, Biotium’s original GelGreen® nucleic acid gel stain (catalog number 41005) is more sensitive for agarose gel applications. PAGE GelGreen® can be removed from DNA after agarose gel staining using commonly available gel extraction kits. PAGE GelGreen® is 2-3 times more sensitive for dsDNA over RNA. For detailed information, please visit the DNA Stain Technology page.

PAGE GelGreen® was subjected to a series of tests at Biotium and two independent laboratories to assess the dye’s safety for routine handling and disposal. Laboratory tests show that the dye is impenetrable to latex or nitrile gloves and cell membranes. Unlike the highly mutagenic EtBr and the reportedly mutation-enhancing SYBR® Green I (1), PAGE GelGreen® is non-toxic and non-mutagenic in bacterial AMES tests at concentrations well above the working concentrations used in gel staining. PAGE GelGreen® successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization, under which it is classified as non-hazardous waste. A complete safety report is available under the downloads tab.

PAGE GelGreen® is a ready-to-use staining solution that is available in 1X in water and 10,000X in water. Also see PAGE GelRed®.

SYBR is a registered trademark of Thermo Fisher Scientific. Dark Reader is a registered trademark of Clare Chemical.

Technology

Molecular Biology

Nucleic Acid Gel Stains

Product Attributes

Size

0.1 mL, 500 uL, 4 L

Format

10,000X in water, 1X in water

Documents, Protocols, SDS and COA

Documents

GelRed, GelGreen & PAGE GelRed Flyer

Protocols

PI-41013

PI-41007

SDS

SDS-41007

SDS-41013

COA

Request the Certificate of Analysis

References

1. Mutation Research 492, 91 (2001).

FAQs

GelRed® and GelGreen® Nucleic Acid Gel Stains

Why does my RNA or ssDNA appear yellowish-orange or pinkish with GelGreen®?

We and other users have often observed that GelGreen® stains ssDNA and RNA orange/ pink and dsDNA green. We have also seen that smaller dsDNA fragments can appear orange-pink, the color ranging from white-pink-orange. We are not sure about the underlying mechanism, possibly the structure of single-stranded nucleic acids favors an altered stacking interaction of GelGreen® monomers leading to the formation of J-aggregates that have red emission.

Can GelRed®/GelGreen® be used to stain DNA in acrylamide gels?

Yes, use the post-staining protocol for polyacrylamide gels. For polyacrylamide gels containing 3.5-10% acrylamide, typical staining time is 30 minutes to 1 hour with gels of higher acrylamide content requiring longer staining time.,

Biotium also offers PAGE GelRed® a non-toxic, non-mutagenic dye specifically designed for staining DNA in polyacrylamide gels.

How much GelRed® or GelGreen® should I add to my 6X DNA loading buffer?

We don’t recommend adding GelRed® or GelGreen® directly to loading buffer, because this can result in inaccurate band migration. Biotium offers 6X GelRed® Prestain Loading Buffers designed for this application, although we do not recommended them for applications where precise DNA band sizing is required. For the most accurate determination of DNA band sizes, we recommend using GelRed® post-staining (see the GelRed® protocol for details).

View more FAQs