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GelGreen® Nucleic Acid Gel Stain

GelGreen® is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. GelGreen® is far more sensitive than SYBR Safe.

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10,000X in water, 10,000X in DMSO

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Product Description

GelGreen® is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. GelGreen® is far more sensitive than SYBR Safe. Unlike SYBR® dyes, which are known to be unstable, GelGreen® is very stable, both hydrolytically and thermally. GelGreen® is compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as blue LED light box, 488 nm laser-based gel scanner, or Dark Reader®).

GelGreen® can be used for either post-electrophoresis gel staining or precast gel staining. For detailed protocols for use, please download the GelGreen® Product Information Sheet. Also see our GelRed® and GelGreen® Frequently Asked Questions (FAQs).

A series of safety tests have confirmed that GelGreen® is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, working strength GelGreen® can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. For detailed test results, you may download a complete GelRed®/GelGreen® Safety Report.

For new users, we recommend GelGreen® 10,000X in water (catalog no. 41005), our latest formulation that eliminates the hazards of handling DMSO for better safety. We continue to offer GelGreen® 10,000X solution in DMSO for established users who do not wish to change their existing laboratory protocols. Also see GelRed® Nucleic Acid Gel Stain, our safer replacement for ethidium bromide.

FEATURES

  • Safer than EB: Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic
  • Easy disposal: Passed environmental safety tests for direct disposal of working solutions down the drain or in regular trash
  • Ultra-sensitive: Much more sensitive than EtBr and SYBR® Safe
  • Extremely stable: Available in water, stable at room temperature for long-term storage and microwavable
  • Simple to use: Very simple procedures for precast or post-electrophoresis gel staining
  • Compatible with a standard UV transilluminator or a gel reader with visible light excitation: Replaces SYBR® or GelStar® with no optical setting change
  • Compatible with downstream applications: Compatible with gel purification, restriction digest, sequencing and cloning.

GelGreen® and GelRed® and their uses are covered by granted and/or pending US and International patents. SYBR is registered trademark of Molecular Probes, Inc; GelStar is a registered trademark of FMC corporation; Dark Reader is a registered trademark of Clare Chemical.

For more information, view our DNA Stain technology page.

References

Download a list of selected References for GelRed® and GelGreen®.

 

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