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GelGreen Nucleic Acid Gel Stain

GelGreen™ is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. GelGreen™ is far more sensitive than SYBR Safe.

100 bp DNA Ladder in TE Buffer
Catalog #

41004, 41005, 41005-1, - 41005-TView allHide

Size

Trial size (0.1 mL), 0.5 mL, 10 mL

Format

10,000X in water, 10,000X in DMSO

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0.5 mL
10 mL
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Product Description

Limited time offer! Free LED Gel Illuminator with purchase of 10 mL GelGreen. Offer code GBFREE.

GelGreen™ is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. GelGreen™ is far more sensitive than SYBR Safe. Unlike SYBR® dyes, which are known to be unstable, GelGreen™ is very stable, both hydrolytically and thermally. GelGreen™ is compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as blue LED light box, 488 nm laser-based gel scanner, or Dark Reader®).

GelGreen™ can be used for either post-electrophoresis gel staining or precast gel staining. For detailed protocols for use, please download the GelGreen™ Product Information Sheet. Also see our GelRed™ and GelGreen™ Frequently Asked Questions (FAQs).

A series of safety tests have confirmed that GelGreen™ is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, working strength GelGreen™ can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. For detailed test results, you may download a complete GelRed/GelGreen Safety Report.

For new users, we recommend GelGreen™ 10,000X in water (catalog no. 41005), our latest formulation that eliminates the hazards of handling DMSO for better safety. We continue to offer GelGreen™ 10,000X solution in DMSO for established users who do not wish to change their existing laboratory protocols. Also see GelRed™ Nucleic Acid Gel Stain, our safer replacement for ethidium bromide.

FEATURES

  • Safer than EB: Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic
  • Easy disposal: Passed environmental safety tests for direct disposal of working solutions down the drain or in regular trash
  • Ultra-sensitive: Much more sensitive than EtBr and SYBR® Safe
  • Extremely stable: Available in water, stable at room temperature for long-term storage and microwavable
  • Simple to use: Very simple procedures for precast or post-electrophoresis gel staining
  • Compatible with a standard UV transilluminator or a gel reader with visible light excitation: Replaces SYBR® or GelStar® with no optical setting change
  • Compatible with downstream applications: Compatible with gel purification, restriction digest, sequencing and cloning.

GelGreen and GelRed and their uses are covered by granted and/or pending US and International patents. SYBR is registered trademark of Molecular Probes, Inc; GelStar is a registered trademark of FMC corporation; Dark Reader is a registered trademark of Clare Chemical.

References

Download a list of selected References for GelRed and GelGreen.

 

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Support & Faq

GelRed™ and GelGreen™ Nucleic Acid Gel Stains

The main difference between GelRed™ and GelGreen™ is their fluorescence excitation and emission wavelengths. GelRed™ has red fluorescence, similar to ethidium bromide. GelGreen™ has green fluorescence, similar to SYBR® Green or SYBR® Safe. Both dyes are compatible with standard UV transilluminators. GelGreen™ is also compatible with blue light transilluminators, which allow users to avoid exposing themselves and their DNA samples to ultraviolet radiation.

GelRed™ and GelGreen™ have higher sensitivity for double stranded nucleic acids compared to single stranded nucleic acids, but GelRed™ is more sensitive for staining single stranded nucleic acids than GelGreen™. GelRed™ is about twice as sensitive for double stranded nucleic acids compared to single-stranded nucleic acids, and about five times more sensitive than GelGreen™ for staining single stranded nucleic acids.

For more information about these products, please visit our DNA stains technology page.

View the GelRed™ Product Line

View the GelGreen™ Product Line

Category: GelRed™ and GelGreen™ Nucleic Acid Gel Stains

← FAQs

The water formulation is a newer and improved product compared to the stock in DMSO. We recommend using dyes in water to avoid the potential hazards of handling DMSO, which can be absorbed through the skin. We continue to offer dyes in DMSO because some users do not wish to alter their established laboratory protocols. Based on internal testing, both formulations perform similarly.

Category: GelRed™ and GelGreen™ Nucleic Acid Gel Stains

← FAQs

GelRed™ is compatible with a standard UV transilluminator (302 or 312 nm).
GelGreen™ has sufficient absorption between 250-300 nm and a strong absorption peak at around 500 nm. GelGreen™ is compatible with a 254 nm UV transilluminator or a gel reader with visible light excitation such as a Dark Reader or a 488 nm laser gel scanner.

Category: GelRed™ and GelGreen™ Nucleic Acid Gel Stains

← FAQs

← FAQs

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.