Support & Resources

+1

6X GelRed® Prestain Loading Buffer with Tracking Dye

6X GelRed® Prestain Loading Buffers are DNA gel loading buffers containing GelRed® for one-step fluorescent staining and gel loading of DNA. Choose loading buffer with two blue tracking dyes or loading buffer with orange tracking dye.

Product Attributes

Tracking dye

Blue tracking dyes, Orange tracking dye

Tracking dye
Size
Catalog #
price
Qty
1 mL
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

Note: An improved version of this product is available. GelRed® Prestain Plus 6X DNA Loading Dye has been reformulated formulated to increase brightness and minimize DNA migration shift.

GelRed® is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr). 6X GelRed® Prestain Loading Buffers are gel loading buffers containing density agents, tracking dyes, and GelRed® dye. The 6X prestain loading buffer is added to samples in place of gel loading buffer, and eliminates the need to add fluorescent DNA dye to the agarose gel during casting. Download the product protocol on this page for a comparison of prestain, precast, and post-stain protocols for GelRed®, and to find the recommended method for your application.

For detailed information, please visit the DNA Stain Technology page.

The 6X GelRed® Prestain Loading Buffers are available with blue tracking dyes or with orange tracking dye. Prestain buffer with blue tracking dyes (cat. no. 41009) contains two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel. Prestain buffer with orange tracking dye (cat. no. 41010) contains an orange electrophoresis tracking dye that runs at approximately 50 bp in a 1% agarose gel.

GelRed® and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed® without changing your existing imaging system. In addition, GelRed® is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed® is compatible with downstream applications such as sequencing and cloning. GelRed® is efficiently removed from DNA by commercial gel extraction kits or by phenol/chloroform extraction and ethanol precipitation.

This product is a replacement for catalog no. 31010.

Product / MethodProcedureAdvantagesDisadvantagesRecommended for
DNA prestaining with GelRed® Prestain Plus 6X DNA Loading DyeGelRed® loading buffer is added directly to the DNA sample before loading• Fast & simple: one-step sample loading & DNA staining

• Less concentrated dye for safer handling

• Can re-run a gel to use empty lanes
• Not recommended for PAGE, DGGE, EMSA, or PFGE gels

• Dye may cause band migration issues when loading larger amounts of DNA (more than ~100 ng/band), or for some restriction digests
• Routine agarose gels

• Recommended loading 50-200 ng ladder or 2-5 uL PCR product ( ~100 ng/band or less)
Precast gel staining with GelRed® 10,000X in waterGelRed® is mixed with molten agarose before gel castingFamiliar protocol, rapid results
Precast gel staining with GelRed® Agarose LE
Agarose is supplied pre-coated with GelRed®, just dissolve, heat, and pourSafer & more convenient, no need to handle concentrated dye
Post-electrophoresis gel staining with GelRed® 10,000X in water
- or -
GelRed® 3X in water
No fluorescent dye is added to the gel, it is stained in 3X GelRed® solution after electrophoresis• Most accurate sizing/sharpest bands

• Staining solution can be re-used

• Enhance sensitivity by adding NaCl
Extra staining step (up to 30 minutes) after electrophoresis (some customers report good results after only 5 minutes if dye is not reused)• Highly accurate band sizing

• Gels with more than ~100 ng DNA per band

• Analyzing restriction digests
Post-electrophoresis gel staining of PAGE gels using PAGE GelRed® 10,000X or 1X in waterNo fluorescent dye is added to the gel, it is stained in 1X PAGE GelRed® solution after electrophoresis• Formulated for efficient penetration and staining of polyacrylamide gels

• Like the classic GelRed®, it is safe and environmentally friendly
Extra staining step of approx. 30 minutes after electrophoresisStaining of nucleic acids in PAGE gels

References

Download a list of selected References for GelRed® and GelGreen®.

1. Journal of Hazardous Materials (2016), http://dx.doi.org/10.1016/j.jhazmat.2017.03.049

 

You may also like…

    DNAzure® Blue Nucleic Acid Gel Stain, 100X

    DNAzure® Blue Nucleic Acid Gel Stain is an ultrasensitive reagent for visible staining of dsDNA in agarose or polyacrylamide gels.

    GelGreen® Nucleic Acid Gel Stain

    GelGreen® is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. GelGreen® is far more sensitive than SYBR Safe.

    GelRed® Nucleic Acid Gel Stain

    GelRed® is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed® is far more sensitive than EB without requiring a destaining step.

    GelRed® Prestain Plus 6X DNA Loading Dye

    6X DNA loading buffer that includes ultra-sensitive, non-toxic GelRed® dye. This is an improved version of our original GelRed® prestain loading buffer, formulated to minimize DNA migration shift.

    Ready-to-Use 1 kb DNA Ladder

    1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The ladder consists of 13 bands that are generated from PCR and restriction enzyme digestion of double stranded DNA.

    Ready-to-Use 100 bp DNA Ladder

    100 bp DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 1500 bp. The 500 bp and 1,500 bp bands contain more DNA to provide internal orientation.