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6X GelRed Prestain Loading Buffer with Tracking Dye

6X GelRed Prestain Loading Buffers are DNA gel loading buffers containing GelRed for one-step fluorescent staining and gel loading of DNA. Choose loading buffer with two blue tracking dyes or loading buffer with orange tracking dye.

100 bp DNA Ladder in TE Buffer
Catalog #

41009, - 41010View allHide

Size

1 mL

Tracking dye

Blue tracking dyes, Orange tracking dye

Tracking dye
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1 mL
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Product Description

GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr). 6X GelRed Prestain Loading Buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. The 6X prestain loading buffer is added to samples in place of gel loading buffer, and eliminates the need to add fluorescent DNA dye to the agarose gel during casting. Download the product protocol on this page for a comparison of prestain, precast, and post-stain protocols for GelRed, and to find the recommended method for your application.

For detailed information, please visit the DNA Stain Technology page.

The 6X GelRed Prestain Loading Buffers are available with blue tracking dyes or with orange tracking dye. Prestain buffer with blue tracking dyes (cat. no. 41009) contains two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel. Prestain buffer with orange tracking dye (cat. no. 41010) contains an orange electrophoresis tracking dye that runs at approximately 50 bp in a 1% agarose gel.

GelRed and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed without changing your existing imaging system. In addition, GelRed is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed is compatible with downstream applications such as sequencing and cloning. GelRed is efficiently removed from DNA by commercial gel extraction kits or by phenol/chloroform extraction and ethanol precipitation.

This product is a replacement for catalog no. 31010.

References

Download a list of selected References for GelRed and GelGreen.

1. Journal of Hazardous Materials (2016), http://dx.doi.org/10.1016/j.jhazmat.2017.03.049

 

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Support & Faq

GelRed™ and GelGreen™ Nucleic Acid Gel Stains

The main difference between GelRed™ and GelGreen™ is their fluorescence excitation and emission wavelengths. GelRed™ has red fluorescence, similar to ethidium bromide. GelGreen™ has green fluorescence, similar to SYBR® Green or SYBR® Safe. Both dyes are compatible with standard UV transilluminators. GelGreen™ is also compatible with blue light transilluminators, which allow users to avoid exposing themselves and their DNA samples to ultraviolet radiation.

GelRed™ and GelGreen™ have higher sensitivity for double stranded nucleic acids compared to single stranded nucleic acids, but GelRed™ is more sensitive for staining single stranded nucleic acids than GelGreen™. GelRed™ is about twice as sensitive for double stranded nucleic acids compared to single-stranded nucleic acids, and about five times more sensitive than GelGreen™ for staining single stranded nucleic acids.

For more information about these products, please visit our DNA stains technology page.

View the GelRed™ Product Line

View the GelGreen™ Product Line

Category: GelRed™ and GelGreen™ Nucleic Acid Gel Stains

← FAQs

The water formulation is a newer and improved product compared to the stock in DMSO. We recommend using dyes in water to avoid the potential hazards of handling DMSO, which can be absorbed through the skin. We continue to offer dyes in DMSO because some users do not wish to alter their established laboratory protocols. Based on internal testing, both formulations perform similarly.

Category: GelRed™ and GelGreen™ Nucleic Acid Gel Stains

← FAQs

GelRed™ is compatible with a standard UV transilluminator (302 or 312 nm).
GelGreen™ has sufficient absorption between 250-300 nm and a strong absorption peak at around 500 nm. GelGreen™ is compatible with a 254 nm UV transilluminator or a gel reader with visible light excitation such as a Dark Reader or a 488 nm laser gel scanner.

Category: GelRed™ and GelGreen™ Nucleic Acid Gel Stains

← FAQs

← FAQs

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.