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6X GelRed® Prestain Loading Buffer with Tracking Dye

6X GelRed® Prestain Loading Buffers are DNA gel loading buffers containing GelRed® for one-step fluorescent staining and gel loading of DNA, with your choice of blue or orange tracking dyes.

Product Attributes

Tracking dye

Blue tracking dyes, Orange tracking dye

Tracking dye
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Product Description

Note: An improved version of this product is available. GelRed® Prestain Plus 6X DNA Loading Dye has been reformulated formulated to increase brightness and minimize DNA migration shift.

This 6X DNA loading dye contains GelRed® fluorescent DNA/RNA dye for convenient one-step loading and staining.

  • 6X loading dye includes GelRed® DNA stain
  • One-step gel loading and DNA staining
  • Your choice of blue or orange visible tracking dyes
  • Highly sensitive, non-mutagenic red fluorescent DNA dye

Convenient One-Step Loading & Staining

The 6X GelRed® Prestain Loading Buffers are available with blue tracking dyes or with orange tracking dye. Prestain buffer with blue tracking dyes (cat. no. 41009) contains two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel. Prestain buffer with orange tracking dye (cat. no. 41010) contains an orange electrophoresis tracking dye that runs at approximately 50 bp in a 1% agarose gel.

A Superior & Safer Alternative to EtBr

GelRed® is a sensitive, stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr). GelRed® and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed® without changing your existing imaging system. In addition, GelRed® is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed® is compatible with downstream applications such as sequencing and cloning. It is efficiently removed from DNA by gel extraction kits or by phenol/chloroform extraction and ethanol precipitation.

GelRed® was subjected to a series of tests at Biotium and by three independent testing services to assess the dye’s safety for routine handling and disposal. Test results confirm that the dye is impenetrable to both latex gloves and cell membranes. The dye is noncytotoxic, nonmutagenic, and classified as non-hazardous for disposal under CCR Title 22 Hazardous Waste Characterization. See the GelRed® and GelGreen® Safety Report. To learn more, see our GelRed® Technology Page and GelRed® FAQs.

How Safe is Your Gel Stain?

Many so-called “safe” DNA dyes like SYBR® Safe, Midori Green, GreenSafe, SafeView™, and RedSafe™ not only have low sensitivity, but also readily penetrate living cells to bind DNA, and some are cytotoxic. Unlike these dyes, GelRed® is cell membrane-impermeant, so it cannot enter living cells to interact with their DNA. See our Gel Stains Comparison Flyer or  Gel Stains Comparison White Paper for details.

Choose the Right Stain for Your Application

Product / MethodProcedureAdvantagesDisadvantagesRecommended for
DNA prestaining with GelRed® Prestain Plus 6X DNA Loading DyeGelRed® loading buffer is added directly to the DNA sample before loading• Fast & simple: one-step sample loading & DNA staining

• Less concentrated dye for safer handling

• Can re-run a gel to use empty lanes
• Not recommended for PAGE, DGGE, EMSA, or PFGE gels

• Dye may cause band migration issues when loading larger amounts of DNA (more than ~100 ng/band), or for some restriction digests
• Routine agarose gels

• Recommended loading 50-200 ng ladder or 2-5 uL PCR product ( ~100 ng/band or less)
Precast staining with GelRed® 10,000X in waterGelRed® is mixed with molten agarose before gel castingFamiliar protocol, rapid results
Precast staining with GelRed® Agarose LE
Agarose is supplied pre-coated with GelRed®, just dissolve, heat, and pourSafer & more convenient, no need to handle concentrated dye
Post-electrophoresis staining with GelRed® 10,000X in water
- or -
GelRed® 3X in water
No fluorescent dye is added to the gel, it is stained in 3X GelRed® solution after electrophoresis• Most accurate sizing/sharpest bands

• Staining solution can be re-used

• Enhance sensitivity by adding NaCl
Extra staining step (up to 30 minutes) after electrophoresis (some customers report good results after only 5 minutes if dye is not reused)• Highly accurate band sizing

• Gels with more than ~100 ng DNA per band

• Analyzing restriction digests
Post-electrophoresis staining of PAGE gels using PAGE GelRed® 10,000X or 1X in waterNo fluorescent dye is added to the gel, it is stained in 1X PAGE GelRed® solution after electrophoresis• Formulated for efficient penetration and staining of polyacrylamide gels

• Like the classic GelRed®, it is safe and environmentally friendly
Extra staining step of approx. 30 minutes after electrophoresisStaining of nucleic acids in PAGE gels

 

GelRed® and its uses are covered by granted and/or pending US and International patents. GelRed and EvaGreen are registered trademarks of Biotium, Inc. SafeView is a trademark of Applied Biological Materials; RedSafe is a trademark of iNtRON Biotechnology. SYBR is a registered trademark of Thermo Fisher Scientific.

References

1. Journal of Hazardous Materials (2017) 333, 285-292. http://dx.doi.org/10.1016/j.jhazmat.2017.03.049
2. J Dairy Sci (2019) 102, 135–139. https://doi.org/10.3168/jds.2018-14733

Download a list of selected References for GelRed® and GelGreen®.

 

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