Note: An improved version of this product is available. GelRed® Prestain Plus 6X DNA Loading Dye has been reformulated formulated to increase brightness and minimize DNA migration shift.
GelRed® is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr). 6X GelRed® Prestain Loading Buffers are gel loading buffers containing density agents, tracking dyes, and GelRed® dye. The 6X prestain loading buffer is added to samples in place of gel loading buffer, and eliminates the need to add fluorescent DNA dye to the agarose gel during casting. Download the product protocol on this page for a comparison of prestain, precast, and post-stain protocols for GelRed®, and to find the recommended method for your application.
For detailed information, please visit the DNA Stain Technology page.
The 6X GelRed® Prestain Loading Buffers are available with blue tracking dyes or with orange tracking dye. Prestain buffer with blue tracking dyes (cat. no. 41009) contains two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel. Prestain buffer with orange tracking dye (cat. no. 41010) contains an orange electrophoresis tracking dye that runs at approximately 50 bp in a 1% agarose gel.
GelRed® and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed® without changing your existing imaging system. In addition, GelRed® is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed® is compatible with downstream applications such as sequencing and cloning. GelRed® is efficiently removed from DNA by commercial gel extraction kits or by phenol/chloroform extraction and ethanol precipitation.
This product is a replacement for catalog no. 31010.
|Product / Method||Procedure||Advantages||Disadvantages||Recommended for
|DNA prestaining with GelRed® Prestain Plus 6X DNA Loading Dye||GelRed® loading buffer is added directly to the DNA sample before loading||• Fast & simple: one-step sample loading & DNA staining|
• Less concentrated dye for safer handling
• Can re-run a gel to use empty lanes
|• Not recommended for PAGE, DGGE, EMSA, or PFGE gels|
• Dye may cause band migration issues when loading larger amounts of DNA (more than ~100 ng/band), or for some restriction digests
|• Routine agarose gels
• Recommended loading 50-200 ng ladder or 2-5 uL PCR product ( ~100 ng/band or less)
|Precast gel staining with GelRed® 10,000X in water||GelRed® is mixed with molten agarose before gel casting||Familiar protocol, rapid results
|Precast gel staining with GelRed® Agarose LE|
|Agarose is supplied pre-coated with GelRed®, just dissolve, heat, and pour||Safer & more convenient, no need to handle concentrated dye
|Post-electrophoresis gel staining with GelRed® 10,000X in water|
- or -
GelRed® 3X in water
|No fluorescent dye is added to the gel, it is stained in 3X GelRed® solution after electrophoresis||• Most accurate sizing/sharpest bands |
• Staining solution can be re-used
• Enhance sensitivity by adding NaCl
|Extra staining step (up to 30 minutes) after electrophoresis (some customers report good results after only 5 minutes if dye is not reused)||• Highly accurate band sizing
• Gels with more than ~100 ng DNA per band
• Analyzing restriction digests
|Post-electrophoresis gel staining of PAGE gels using PAGE GelRed® 10,000X or 1X in water||No fluorescent dye is added to the gel, it is stained in 1X PAGE GelRed® solution after electrophoresis||• Formulated for efficient penetration and staining of polyacrylamide gels|
• Like the classic GelRed®, it is safe and environmentally friendly
|Extra staining step of approx. 30 minutes after electrophoresis||Staining of nucleic acids in PAGE gels