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DNAzure Blue Nucleic Acid Gel Stain, 100X

DNAzure™ Blue Nucleic Acid Gel Stain, 100X is an ultrasensitive reagent for visible staining of dsDNA in agarose gels or polyacrylamide gels.

DNAazure diagonal gels
Catalog #

41020

Size

10 mL

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10 mL
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Product Description

Visualize DNA bands in gels by eye, with detection sensitivity rivaling most fluorescence-based gel stains.

Key to the technology is a DNA-binding dye that turns from colorless to deep blue upon exposure to light.

The benefits include:

  • Deep blue bands visible by the naked eye following 5-30 min light exposure
  • Ultrasensitive detection, as little as ~1 ng DNA
  • Simplified DNA band excision, without the need for DNA damaging UV light
  • Compatible with downstream applications such as sequencing and cloning
  • Expensive gel documentation systems not required for imaging

 

 

 

 

 

 

 

 

Biotium’s 1 kb DNA ladder was loaded on a 1% agarose gel in two-fold dilutions, ranging from 200 ng to 25 ng total ladder per lane. The mass of the 500 bp band in each lane is labeled. The gel was stained with DNAzure™ Blue Nucleic Acid Gel Stain for 25 minutes, and then the visible blue DNA bands were developed for 30 minutes using the Glo-Plate™ Blue LED transilluminator. The gel was placed on a white light transilluminator and imaged with a cell phone camera.

 

After incubation with DNAzure Blue Nucleic Acid Gel Stain, the gel must be exposed to bright light to facilitate the development of dark blue DNA bands. 

Light exposure can be performed with a variety of white and blue light sources. For best results, we recommend performing the light exposure with either the Gel-Bright™ LED Gel Illuminator or the Glo-Plate™ Blue LED Illuminator. Both devices produce blue light from LEDs and are very effective at rapidly producing the color change in DNAzure™-stained gels. The Gel-Bright™ is also an excellent instrument for viewing gels stained with the fluorescent green DNA gel stain, GelGreen™.

 

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    GelRed Nucleic Acid Gel Stain

    GelRed™ is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed™ is far more sensitive than EB without requiring a destaining step.

    GelGreen Nucleic Acid Gel Stain

    GelGreen™ is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. GelGreen™ is far more sensitive than SYBR Safe.

    Ready-to-Use 1 kb DNA Ladder

    1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The ladder consists of 13 bands that are generated from PCR and restriction enzyme digestion of double stranded DNA.

    Ready-to-Use 100 bp DNA Ladder

    100 bp DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 1500 bp. The 500 bp and 1,500 bp bands contain more DNA to provide internal orientation.

    Gel-Bright LED Gel Illuminator

    The Gel-Bright™ LED Gel Illuminator is a non-UV lightbox for detection of fluorescently labeled nucleic acids and protein.

    Glo-Plate Blue LED Illuminator

    The Glo-Plate™ Blue LED Illuminator is a multi-functional blue LED light box. It can be used for photolysis of PMAxx™ or PMA in viability PCR. It is also an excellent way to develop the staining of gels stained with the visible blue DNA gel stain DNAzure™.

Support & Faq

DNAzure™ visible DNA gel stain

For optimal staining, we recommend diluting the 100X DNAzure™ stock to 1X working solution fresh each time. However, if a 1X solution stored at 4C and protected from light, the gel stain should to be good up to two weeks.

Category: DNAzure™ visible DNA gel stain

← FAQs

Yes, the 1X DNAzure™ staining solution can be re-used for multiple gels, under certain conditions. Importantly, the staining solution must be removed from the gel before the gel is exposed to light to develop the bands. If the staining solution undergoes the light exposure, it will not be able to stain another gel. The used staining solution should be stored in the refrigerator, protected from light, between uses. We have successfully re-used the staining solution stored for up to 2 weeks, and used up to 4 times with little loss of signal (after 5 or 6 uses, the sensitivity was noticeably lower).

Category: DNAzure™ visible DNA gel stain

← FAQs

We recommend storing DNAzure™ at 4C. After storage at room temperature, we have seen some loss of dye stability.

Category: DNAzure™ visible DNA gel stain

← FAQs

← FAQs

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.