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DNAzure® Blue Nucleic Acid Gel Stain, 100X

A blue nucleic acid gel stain to visualize dsDNA in agarose or polyacrylamide gels by the unaided eye.

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DNAzure® Blue Nucleic Acid Gel Stain, 100X
DNAzure® Blue Nucleic Acid Gel Stain, 100X - Image 2
DNAzure vs GelRedComparison of DNAzure® visible DNA staining with GelRed® fluorescent DNA staining. Biotium’s 1 kb DNA ladder was loaded on a 1% agarose gel in two-fold dilutions, ranging from 200 ng to 25 ng total ladder per lane. The mass of the 500 bp band in each lane is labeled. The gel on the left was stained with DNAzure® Blue Nucleic Acid Gel Stain for 25 minutes, and then the visible blue DNA bands were developed for 30 minutes using a Glo-Plate™ Blue LED Illuminator. The gel was placed on a white light box and imaged with a cell phone camera. The gel on the right was stained with 3X GelRed® Nucleic Acid Gel Stain for 60 minutes. The gel was imaged with a UVP GelDoc-It® Imaging System using a UV transilluminator and EtBr filter.
DNAzure® Blue Nucleic Acid Gel Stain, 100X - Image 4Comparison of visible and near-infrared fluorescence detection of the same DNAzure®stained gel. Biotium’s Ready-to-Use 1 kb DNA ladder was loaded on a 1% agarose gel in two-fold dilutions, ranging from 200 ng to 3.125 ng total ladder per lane. The mass of the 1500 bp band (marked by arrow) in each lane is labeled. The gel was stained with DNAzure® Blue Nucleic Acid Gel Stain for 30 minutes, and then the visible blue DNA bands were developed for 30 minutes using a white LED lamp. Left: Visible blue bands imaged on a UVP GelDoc-It® Imaging System using a UV transilluminator with a white light converter plate and a Visi-Blue™ filter. Right: Near-infrared fluorescence imaged on a LI-COR® Odyssey® near-infrared imaging system in the 700 nm channel. The gel was imaged face-down with gain set to 8. DNAzure®-stained bands also fluoresce in the Odyssey® 800 nm channel (not shown). This gel was stored in staining buffer on the benchtop for six weeks before these images were acquired.
DNAzure® Blue Nucleic Acid Gel Stain, 100X - Image 5Biotium’s 1 kb DNA ladder was loaded on a 10% acrylamide/1X TBE gel in two-fold dilutions, ranging from 200 ng to 25 ng total ladder per lane, then the gel was stained with 1X DNAzure® Blue Nucleic Acid Gel Stain for 25 minutes in the dark. The visible blue DNA bands were developed by exposing the gel to light using a Gel-Bright™ LED Illuminator for the time indicated, then the gel was placed on a white light box and photographed.

Product Description

Check out DNAzure® 2.0 Visible Blue DNA Gel Stain Kit, a new and improved formulation of the original DNAzure® Blue Nucleic Acid Gel Stain that delivers enhanced sensitivity for visible staining of dsDNA in both agarose and polyacrylamide gels.

Visualize DNA bands in gels by unaided eye and without UV light sources. Detection is highly sensitive and rivals most fluorescence-based gel stains.

  • Deep blue bands visible by the naked eye following 5-30 min bright light exposure
  • Safer non-UV light sources eliminate the need for protective eye wear and expensive imaging equipment
  • Ultrasensitive detection, as little as ~1 ng DNA
  • Simplified DNA band excision, without the need for DNA-damaging UV light
  • Bands are stable for weeks after color development
  • Compatible with downstream applications such as sequencing and cloning
  • Stain also can be imaged on LI-COR® Odyssey® or other near-IR imaging systems

DNAzure® Blue Nucleic Acid Gel Stain is a DNA-binding dye that turns from colorless to deep blue upon exposure to bright light. After color development, the stain also has broad emission near-infrared fluorescence that can be imaged using the LI-COR®, Odyssey®, or similar near-IR imaging systems. The sensitivity of detection is similar for visible color and near-IR imaging. DNAzure® is compatible with agarose gels or polyacrylamide gels as well as downstream applications such as sequencing and cloning. The dye efficiently removed from DNA by common gel extraction kits that utilize silica-based DNA purification columns.

 

 

 

 

 

 

 

 

Biotium’s 1 kb DNA ladder was loaded on a 1% agarose gel in two-fold dilutions, ranging from 200 ng to 25 ng total ladder per lane. The mass of the 500 bp band in each lane is labeled. The gel was stained with DNAzure® Blue Nucleic Acid Gel Stain for 25 minutes, and then the visible blue DNA bands were developed for 30 minutes using the Glo-Plate™ Blue LED Illuminator. The gel was placed on a white light box and imaged with a cell phone camera.

 

After incubation with DNAzure® Blue Nucleic Acid Gel Stain, the gel must be exposed to bright light to facilitate the development of dark blue DNA bands. 

 

Light exposure can be performed with a variety of white and blue light sources. For best results, we recommend performing the light exposure with the Glo-Plate™ White Photoactivation Device or  Glo-Plate™ 2.0 Blue LED Illuminator.

Choose the Right Stain for Your Application

We recommend DNAzure® 2.0 Visible Blue DNA Gel Stain Kit as an improved alternative to the original DNAzure® Blue Nucleic Acid Gel Stain. The improved formulation delivers enhanced sensitivity for visible staining of dsDNA in both agarose and polyacrylamide gels. Also learn about the Original GelRed® Nucleic Acid Gel Stain or GelGreen® Nucleic Acid Gel Stain. GelRed® 3X in water is ready-to-use for post-electrophoresis gel staining, and is supplied in a 4L Cubitainer®. Higher concentrations of Original GelRed® are available as 10,000X in water or DMSO. We also offer GelRed® Agarose and GelRed® Prestain Plus 6X Loading Dye. GelGreen® Nucleic Acid Gel Stain is a safer replacement for SYBR® gel stains and is compatible with visible light excitation. Our Go-Go™ Fast DNA Gel Running Buffer allows running gels 3X faster than with TAE or TBE buffer.

Product / MethodProcedureAdvantagesDisadvantagesRecommended for
DNA staining with EMBER™ Ultra DNA Gel KitAgarose is supplied pre-coated with EMBER™ Ultra Dye, just dissolve, heat, and pour.• Safer and more convenient, no need to handle concentrated dye

• Superior sensitivity, detect as little as ≤1 ng DNA

• No need for post-electrophoresis staining

• Optimal for blue LED gel imagers		• Not suitable for PAGE, DGGE, EMSA, or PFGE gels

• Dye may cause band migration issues when loading larger amounts of DNA (more than ~200 ng/band), or for some restriction digests		• Routine agarose gels
RNA staining with EMBER™ Ultra RNA Gel KitAgarose is supplied pre-coated with EMBER™ Ultra Dye, just dissolve, heat, and pour.• Safer and more convenient stain for RNA, no need to handle concentrated dye

• Superior sensitivity, detect as little as ≤5 ng RNA

• No need for post-electrophoresis staining

• Included loading dye contains formamide for denaturing

• Optimal for blue LED gel imagers		• Will stain DNA as well as RNA

• Dye may cause band migration issues when loading larger amounts of RNA (more than ~200 ng/band)		• Routine RNA gel electrophoresis

• Evaluate total RNA integrity and DNA contamination
DNA prestaining with GelRed® Prestain Plus 6X DNA Loading DyeGelRed® loading buffer is added directly to the DNA sample before loading• Fast & simple: one-step sample loading & DNA staining

• Less concentrated dye for safer handling

• Can re-run a gel to use empty lanes
• Not recommended for PAGE, DGGE, EMSA, or PFGE gels

• Dye may cause band migration issues when loading larger amounts of DNA (more than ~100 ng/band), or for some restriction digests		• Routine agarose gels

• Recommended loading 50-200 ng ladder or 2-5 uL PCR product ( ~100 ng/band or less)
Precast staining with GelRed® 10,000X in water or GelGreen® 10,000X in water

GelRed® or GelGreen® is mixed with molten agarose before gel castingFamiliar protocol, rapid results
Precast staining with GelRed® Agarose LE or GelGreen® Agarose LE
Agarose is supplied pre-coated with GelRed® or GelGreen®, just dissolve, heat, and pourSafer & more convenient, no need to handle concentrated dye
Post-electrophoresis staining with GelRed® 10,000X in water or GelGreen® 10,000X in water
- or -
GelRed® 3X in water
No fluorescent dye is added to the gel, it is stained in 3X GelRed® or 3X GelGreen® solution after electrophoresis• Most accurate sizing/sharpest bands

• Staining solution can be re-used

• Enhance sensitivity by adding NaCl		Extra staining step (up to 30 minutes) after electrophoresis (some customers report good results after only 5 minutes if dye is not reused)• Highly accurate band sizing

• Gels with more than ~100 ng DNA per band

• Analyzing restriction digests
Post-electrophoresis staining with DNAzure® 2.0 Visible Blue DNA Gel Stain KitNo fluorescent dye is added to the gel, it is stained in DNazure® 2.0 solution and then exposed to a bright light source to generate visible blue DNA bands.

We recommend the Glo-Plate™ White Photoactivation Device as a light source for developing DNAzure® 2.0-stained gels		• Allows visualization of DNA bands by the naked eye, no need for a UV light source

• Detect as little as ~1 ng DNA

• Stained bands are stable in gel for weeks

• Also emits near-IR fluorescence (~700 nm) for detection on near-IR imaging systems		Extra staining step (up to 30 minutes) followed by a light exposure step (up to 30 minutes) to generate visible blue DNA bands

• Routine DNA agarose gels

• Visualizing gels without the UV light or expensive imaging systems

• Recommended loading 50-200 ng DNA per lane
Post-electrophoresis staining of PAGE gels using PAGE GelRed® 10,000X or 1X in waterNo fluorescent dye is added to the gel, it is stained in 1X PAGE GelRed® solution after electrophoresis• Formulated for efficient penetration and staining of polyacrylamide gels

• Like the classic GelRed®, it is safe and environmentally friendly		Extra staining step of approx. 30 minutes after electrophoresisStaining of nucleic acids in PAGE gels
GelRed, GelGreen, EvaGreen, and DNAzure are registered trademarks of Biotium, Inc. LI-COR and Odyssey are registered trademarks of LI-COR Inc.

Product Attributes

Size
10 mL
DNA/RNA dye
DNA dye
Storage Conditions
Store at 2 to 8 °C, Protect from light
Assay type/options
DNA/RNA gel staining

Documents, Protocols, SDS and COA

Citations

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FAQs

DNAzure® Visible DNA Gel Stain

We have tested a variety of different light sources for the development of DNAzure® bands in an agarose gel. We have found that most light sources will work, but how long the development takes is dependent on the brightness of the light. The fastest band development is seen with our Glo-Plate™ White Photoactivation Device and Glo-Plate™ 2.0 Blue LED Illuminator. We have also seen good results with other bright, white LED lights. Other light sources that work but take more time include an LED desk lamp and a cell phone light. We found that a 600W halogen lamp did not work well- it was too hot, which melted the gel.

We recommend storing DNAzure® at 4°C. After storage at room temperature, we have seen some loss of dye stability.

Yes, the 1X DNAzure® staining solution can be re-used for multiple gels, under certain conditions. Importantly, the staining solution must be removed from the gel before the gel is exposed to light to develop the bands. If the staining solution undergoes the light exposure, it will not be able to stain another gel. The used staining solution should be stored in the refrigerator, protected from light, between uses. We have successfully re-used the staining solution stored for up to 2 weeks, and used up to 4 times with little loss of signal (after 5 or 6 uses, the sensitivity was noticeably lower).

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