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DNAzure® Blue Nucleic Acid Gel Stain, 100X

DNAzure® Blue Nucleic Acid Gel Stain is an ultrasensitive reagent for visible staining of dsDNA in agarose or polyacrylamide gels.

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10 mL
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Product Description

Visualize DNA bands in gels by eye, with detection sensitivity rivaling most fluorescence-based gel stains. Key to the technology is a DNA-binding dye that turns from colorless to deep blue upon exposure to light.

The benefits include:

    • Deep blue bands visible by the naked eye following 5-30 min light exposure
    • Ultrasensitive detection, as little as ~1 ng DNA
    • Simplified DNA band excision, without the need for DNA-damaging UV light
    • Bands are stable for weeks after color development
    • Compatible with downstream applications such as sequencing and cloning
    • Expensive gel documentation systems not required for imaging
  • Stain also can be imaged on LI-COR® Odyssey® or other near-IR imaging systems









Biotium’s 1 kb DNA ladder was loaded on a 1% agarose gel in two-fold dilutions, ranging from 200 ng to 25 ng total ladder per lane. The mass of the 500 bp band in each lane is labeled. The gel was stained with DNAzure® Blue Nucleic Acid Gel Stain for 25 minutes, and then the visible blue DNA bands were developed for 30 minutes using the Glo-Plate™ Blue LED transilluminator. The gel was placed on a white light transilluminator and imaged with a cell phone camera.


After incubation with DNAzure® Blue Nucleic Acid Gel Stain, the gel must be exposed to bright light to facilitate the development of dark blue DNA bands. 

Light exposure can be performed with a variety of white and blue light sources. For best results, we recommend performing the light exposure with either the Gel-Bright™ LED Gel Illuminator or the Glo-Plate™ Blue LED Illuminator. Both devices produce blue light from LEDs and are very effective at rapidly producing the color change in DNAzure®-stained gels. The Gel-Bright™ is also an excellent instrument for viewing gels stained with the fluorescent green DNA gel stain, GelGreen®.

LI-COR and Odyssey are registered trademarks of LI-COR Inc.

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    GelGreen® Nucleic Acid Gel Stain

    GelGreen® is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. GelGreen® is far more sensitive than SYBR Safe.

    Ready-to-Use 1 kb DNA Ladder

    1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The ladder consists of 13 bands that are generated from PCR and restriction enzyme digestion of double stranded DNA.

    Ready-to-Use 100 bp DNA Ladder

    100 bp DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 1500 bp. The 500 bp and 1,500 bp bands contain more DNA to provide internal orientation.

    Gel-Bright™ LED Gel Illuminator

    The Gel-Bright™ LED Gel Illuminator is a non-UV lightbox for detection of fluorescently labeled nucleic acids and protein.

    Glo-Plate™ Blue LED Illuminator

    The Glo-Plate™ Blue LED Illuminator is a multi-functional blue LED light box. It can be used for photolysis of PMAxx™ or PMA in viability PCR. It is also an excellent way to develop the staining of gels stained with the visible blue DNA gel stain DNAzure®.