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Agarose LE, Ultra-Pure Molecular Biology Grade

High performance agarose for nucleic acid electrophoresis (analytical or preparative) and blotting. Validated for use with Biotium’s GelRed®, GelGreen®, and DNAzure® gel stains.

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Product Description

Ultra-Pure LE Agarose can be used for all routine electrophoresis applications. This agarose has excellent performance for analytical or preparative nucleic acid electrophoresis and blotting.

  • High quality, molecular biology grade agarose
  • Excellent DNA resolution with low background
  • No detectable DNase/RNase activity
  • Ideal for all routine electrophoresis and blotting applications

It has low electroendosmosis (EEO) which confers high electrophoretic mobility. Ultra-pure LE Agarose has high strength and shows low background after staining with common nucleic acid dyes. Each lot is validated for use with Biotium’s GelRed®GelGreen®, and DNAzure® gel stains.

High Quality and High Performance

Biotium’s Ultra-Pure LE Agarose has been evaluated to meet the follows specifications; moisture, ash and sulfate content, electroendosmosis, clarity, gel strength, gelling temperature, melting temperature, and DNase/RNase activity. See Sidebar or PI for details.

Also see our GelRed® Agarose and GelGreen® Agarose, precoated with non-toxic, non-mutagenic GelRed® Dye or GelGreen® Dye, for greater safety and convenience. Also learn about our Go-Go™ Fast DNA Gel Running Buffer, a novel low ionic strength running buffer that allows you to run DNA agarose gels at higher voltage to get results up to 3X faster than with TAE or TBE buffer, streamlining routine DNA gel analysis.

Product Attributes

Storage Conditions
Store at room temperature

Documents, Protocols, SDS and COA

References
  1. Infect Drug Resist, 13, 333, (2020), DOI: 10.2147/IDR.S234295
  2. J. Mar. Aquat. Sci, 5, 142, (2019), DOI: 10.24843/jmas.2019.v05.i01.p17
  3. Fungal ecology, 31, 9, (2018), DOI: 10.1016/j.funeco.2017.10.003
  4. Revista MVZ Córdoba, 23, 6778, (2018), DOI: 10.21897/rmvz.1367
  5. Parasitology, 144, 730, (2017), DOI: 10.1017/S003118201600250X
  6. J Microbiol, 10, e14378, (2017), DOI: 10.5812/jjm.14378

Citations

  1. Infect Drug Resist, 13, 333, (2020), DOI: 10.2147/IDR.S234295
  2. J. Mar. Aquat. Sci, 5, 142, (2019), DOI: 10.24843/jmas.2019.v05.i01.p17
  3. Fungal ecology, 31, 9, (2018), DOI: 10.1016/j.funeco.2017.10.003
  4. Revista MVZ Córdoba, 23, 6778, (2018), DOI: 10.21897/rmvz.1367
  5. Parasitology, 144, 730, (2017), DOI: 10.1017/S003118201600250X
  6. J Microbiol, 10, e14378, (2017), DOI: 10.5812/jjm.14378

FAQs

GelRed® and GelGreen® Nucleic Acid Gel Stains

We and other users have often observed that GelGreen® stains ssDNA and RNA orange/ pink and dsDNA green. We have also seen that smaller dsDNA fragments can appear orange-pink, the color ranging from white-pink-orange. We are not sure about the underlying mechanism, possibly the structure of single-stranded nucleic acids favors an altered stacking interaction of GelGreen® monomers leading to the formation of J-aggregates that have red emission.

Yes, use the post-staining protocol for polyacrylamide gels. For polyacrylamide gels containing 3.5-10% acrylamide, typical staining time is 30 minutes to 1 hour with gels of higher acrylamide content requiring longer staining time.,

Biotium also offers PAGE GelRed® a non-toxic, non-mutagenic dye specifically designed for staining DNA in polyacrylamide gels.

We don’t recommend adding GelRed® or GelGreen® directly to loading buffer, because this can result in inaccurate band migration. Biotium offers 6X GelRed® Prestain Loading Buffers designed for this application, although we do not recommended them for applications where precise DNA band sizing is required. For the most accurate determination of DNA band sizes, we recommend using GelRed® post-staining (see the GelRed® protocol for details).

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