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GelGreen® Agarose LE

GelGreen® Agarose LE is  pre-coated with GelGreen® Nucleic Acid Gel Stain for simple preparation of precast gels. With no need to handle concentrated fluorescent dye while preparing your gel, GelGreen® Agarose offers greater convenience and safety.

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Product Description

GelGreen® Agarose LE is our ultra-pure molecular biology grade LE agarose pre-coated with GelGreen® Nucleic Acid Gel Stain. With GelGreen® Agarose, there is no need to handle concentrated fluorescent dye while preparing your gel, for greater convenience and safety.

  • Non-toxic GelGreen® DNA/RNA dye is already in the agarose
  • No need to handle concentrated dye solutions, for enhanced safety
  • Use with TAE or TBE for 0.8% to 2% gels
  • Low EEO, ultrapure molecular biology grade agarose
  • Compatible with blue light illuminators

GelGreen® Agarose LE has low electroendosmosis (EEO) for high electrophoretic mobility. This agarose has excellent performance for analytical or preparative nucleic acid electrophoresis and blotting. It is suitable for preparing 0.8%-2% gels in TAE or TBE buffer. In a 1% GelGreen® Agarose gel, the final GelGreen® concentration is 1X, just like in our standard precast protocol. GelGreen® Agarose also gives excellent results at percentages between 0.8% (0.8X GelGreen®) up to 2% (2X GelGreen®).

GelGreen® is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye specifically designed for gel staining. GelGreen® is far more sensitive than SYBR® Safe. Unlike SYBR® dyes, which are known to be unstable, GelGreen® is very stable, both hydrolytically and thermally. GelGreen® has UV absorption between 250 nm and 300 nm and a strong absorption peak centered around 500 nm. Thus, GelGreen® is compatible with UV transilluminators or blue light illuminators (like the Dark Reader® or Biotium’s Gel-Bright™ LED Illuminator). With blue light illuminators, researchers can avoid exposure to UV irradiation for themselves and their DNA samples, for a safer work environment and higher cloning efficiency.

Gel staining with GelGreen® is compatible with downstream applications such as sequencing and cloning. GelGreen® can be removed from DNA using a gel extraction kit, or by phenol/chloroform extraction followed by ethanol precipitation. GelGreen® can be used to stain dsDNA, ssDNA or RNA, however GelRed® is much more sensitive for staining single-stranded nucleic acids than GelGreen®. To learn more about GelGreen®, see the GelRed® & GelGreen® technology page, or FAQs (below).

GelGreen® was subjected to a series of tests at Biotium and by three independent testing services to assess the dye’s safety for routine handling and disposal. Test results confirm that the dye is impenetrable to both latex gloves and cell membranes. The dye is non-cytotoxic and non-mutagenic at concentrations well above the working concentrations used in gel staining. Using GelGreen® Agarose further minimizes risk by avoiding the need to handle concentrated dye solution. GelGreen® successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization, under which GelGreen® is classified as non-hazardous waste. See the GelRed® and GelGreen® Safety Report.

We also offer GelRed® Agarose LE and unlabeled Agarose LE, Ultrapure Molecular Biology Grade.

Dark Reader is a registered trademark of Clare Chemical. SYBR is a registered trademark of Thermo Fisher Scientific.

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Support & Faq

GelRed® and GelGreen® Nucleic Acid Gel Stains

The main difference between GelRed® and GelGreen® is their fluorescence excitation and emission wavelengths. GelRed® has red fluorescence, similar to ethidium bromide. GelGreen® has green fluorescence, similar to SYBR® Green or SYBR® Safe. Both dyes are compatible with standard UV transilluminators. GelGreen® is also compatible with blue light transilluminators, which allow users to avoid exposing themselves and their DNA samples to ultraviolet radiation.

GelRed® and GelGreen® have higher sensitivity for double stranded nucleic acids compared to single stranded nucleic acids, but GelRed® is more sensitive for staining single stranded nucleic acids than GelGreen®. GelRed® is about twice as sensitive for double stranded nucleic acids compared to single-stranded nucleic acids, and about five times more sensitive than GelGreen® for staining single stranded nucleic acids.

For more information about these products, please visit our DNA stains technology page.

View the GelRed® Product Line

View the GelGreen® Product Line

Category: GelRed® and GelGreen® Nucleic Acid Gel Stains

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The water formulation is a newer and improved product compared to the stock in DMSO. We recommend using dyes in water to avoid the potential hazards of handling DMSO, which can be absorbed through the skin. We continue to offer dyes in DMSO because some users do not wish to alter their established laboratory protocols. Based on internal testing, both formulations perform similarly.

Category: GelRed® and GelGreen® Nucleic Acid Gel Stains

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GelRed® is compatible with a standard UV transilluminator (302 or 312 nm).
GelGreen® has sufficient absorption between 250-300 nm and a strong absorption peak at around 500 nm. GelGreen® is compatible with a 254 nm UV transilluminator or a gel reader with visible light excitation such as a Dark Reader or a 488 nm laser gel scanner.

Category: GelRed® and GelGreen® Nucleic Acid Gel Stains

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GelRed® and GelGreen® troubleshooting

Many customers use GelRed® or GelGreen® precast gels for convenience. However, because GelRed® and GelGreen® are high affinity dyes designed to be larger dyes to improve their safety, they can affect the migration of DNA in precast gels. Some samples, such as restriction digested DNA may migrate abnormally in GelRed® or GelGreen® precast gels.

Tip #1: Load less DNA

Smearing and smiling in GelRed® or GelGreen® precast gels most often caused by overloading of DNA. If you see band migration shifts or smearing and smiling, try reducing the amount of DNA loaded. The recommended loading amount for ladders and samples of known concentration is 50-200 ng/lane. For samples of unknown concentration, try loading one half or one third of the usual amount of DNA. This usually solves band migration problems.

Tip #2: Try the post-staining protocol

To avoid any interference the dye may have on DNA migration, we recommend using the post-staining protocol. If your application requires loading more than the recommended amount of DNA, use the post-staining protocol. While we recommend post-staining gels for 30 minutes, you may be able see bands in as little as five minutes, depending on how much DNA is present. Post-staining solutions can be reused. See the GelRed® Product Information Sheet or GelGreen® Product Information Sheet for detailed protocols.

Other tips to improve agarose gel resolution:

  • If you see DNA migration issues or smearing after post-staining with GelRed® or GelGreen®, then the problem is not caused by the nucleic acid dye. Avoid overfilling gel wells to prevent smearing of DNA down the surface of the gel.
  • Pour a lower percentage agarose gel. Higher molecular weight DNA separates better with a lower percentage gel.
  • Change the running buffer. TBE buffer has a higher buffering capacity than TAE buffer.

Category: GelRed® and GelGreen® troubleshooting, GelRed® and GelGreen® Nucleic Acid Gel Stains

← FAQs

There are a few possibilities:

  1. The dye may have precipitated out of solution.
    • Heat the GelRed® or GelGreen® solution to 45-50°C for two minutes and vortex to dissolve.
    • Store dye at room temperature to avoid precipitation.
  2. If you are seeing high background staining of the gel, the agarose that you are using may be of low quality. Contaminants in the agarose may bind to the dye, resulting in increased background.

Category: GelRed® and GelGreen® troubleshooting, GelRed® and GelGreen® Nucleic Acid Gel Stains

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