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CF® Dye & Biotin SE Protein Labeling Kits

CF® Dye & Biotin SE Protein Labeling Kits

Kits for succinimidyl ester labeling antibodies or other proteins with Biotium’s next-generation CF® dyes or biotin. 3 x 1 mg labeling reactions per kit.

CF® Dye & Biotin SE Protein Labeling Kits
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FAQs

CF® Dyes
Do you offer alternatives to Akoya Opal™ tyramides?

See below for our recommended alternatives to Akoya tyramides. Unless noted as a direct replacement, these tyramide dyes are spectrally similar alternatives. They have not been validated as direct replacements for use with Akoya kits or imaging systems.

CF® Dye Alternatives for Akoya Tyramides

Akoya TyramideEx/Em (nm)Biotium TyramideEx/Em (nm)Biotium Cat. No.NotesCF® Dye Features
TSA Coumarin402/443CF®405S404/43192197Much brighter & more
photostable alternative		CF®405S Features
Opal™ Polaris 480450/500CF®430426/49896053Recommended
for abundant targets		CF®430 Features
Opal™520494/525CF®488A490/51592171CF®488A Features
TSA Fluorescein494/517Fluorescein492/51496018Direct replacementSee CF®488A for a brighter &
much more photostable alternative
TSA TMR
TSA Cyanine 3		550/570Cyanine 555555/56596020Direct replacement
for Cyanine 3		See CF®555 & CF®568 for brighter
& more photostable alternatives.
CF®555555/56596021Brighter & more
photostable alternatives		CF®555 Features
CF®568562/58392173CF®568 Features
Opal™570550/570CF®550R551/57796077CF®550R Features
CF®555555/565
96021CF®555 Features
TSA Plus Cyanine 3.5581/596CF®583R586/60996085Brighter & more
photostable alternatives		CF®583R Features
CF®594593/61492174CF®594 Features
Opal™620588/616CF®583R586/60996085CF®583R Features
TSA Cyanine 5648/667CF®640R642/66292175Brighter & more
photostable alternative		CF®640R Features
CF®647650/665 96022Brighter alternativeCF®647 Features
CF®660R663/68292195Less cross-talk with visible red dyes;
bright & extremely photostable		CF®660R Features
TSA Plus Cyanine 5.5
Opal™690		673/692
676/694		CF®680R680/70192196Bright & extremely
photostable		CF®680R Features
Opal™ Polaris 780750/770CF®754745/78696090Recommended
for abundant targets		Unique NIR dye for TSA
TSA BiotinN/ABiotin-XXN/A92176Direct replacement
TSA Plus DNPN/ADNPN/A96019Direct replacement
TSA Plus DIGN/AN/AN/AN/AN/A
Note: The CF® Dyes listed here are spectrally similar alternatives to Opal™ dyes, they have not been validated as direct replacements for use with Akoya's kits or imaging system. Opal is a trademark of Akoya Biosciences.

 


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My product was accidentally left out at room temperature or exposed to light. Is it ruined?

Most of our products are stable at room temperature for many days, so in all likelihood the product will still work just fine. To be on the safe side, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.

One exception that we are aware of is GelGreen™, which is more sensitive to light exposure than most of our other fluorescent dyes. If GelGreen™ is exposed to ambient light for a prolonged period of time (days to weeks), its color will change from dark orange to brick red. If this occurs, the GelGreen will no longer work for gel staining.

 


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Which CF® Dye Bungarotoxin will have the lowest background?

Dyes that carry multiple negative charges can introduce background. Usually, this is more of a concern with labeled antibodies that carry many dyes, as opposed to a small toxin like bungarotoxin. When staining tissues, the endogenous autofluorescence of the tissue itself is often the most significant source of background. Endogenous fluorescence background in tissue is usually highest in the blue wavelengths (DAPI channel) and lowest in the far-red (Cy®5 channel). Our CF®633 bungarotoxin (catalog no. 00009) is a far-red conjugate for the Cy®5 channel with a low negative charge that should have low background from either the dye or autofluorescence.

We test fluorescent bungarotoxin on rat skeletal muscle sections. While the tissue shows autofluorescence, the bungarotoxin staining of motor endplates is usually much brighter than the background for all of the dye colors we’ve tested.  However, if you are staining human tissue (especially brain), lipofuscin autofluorescence may be bright in all channels. This usually shows up as bright, punctate dots around cell nuclei. While we would usually recommend our TrueBlack® lipofuscin quenchers for human brain tissue, they are not compatible with bungarotoxin staining. We have, however, found that EverBrite TrueBlack® Mounting Medium (cat. no. 23017) can be used to mount skeletal muscle sections stained with bungarotoxin.

Cy Dye is a registered trademark of Cytiva.


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Product Description

This kit contains reagents for labeling antibodies or other proteins with a fluorescent CF® dye succinimidyl ester (CF® dye SE) or biotin succinimidyl ester (biotin SE). Learn more about next-generation CF® dyes with superior brightness, photostability, and water solubility compared to other fluorescent dyes.

Features

  • 3 labeling reactions of 1 mg each
  • Choice of biotin or CF® dyes ranging from UV to near-IR
  • Includes buffers & ultrafiltration vials for labeling & purification

Kit Components

  • Lyophilized CF® Dye SE or Biotin SE (3 vials)
  • Anhydrous DMSO
  • Sodium Bicarbonate Solution, pH 8.3
  • 1X PBS, pH 7.4
  • Ultrafiltration Vials, 10K MWCO (3 vials)
  • Reaction Vials (3 vials)
  • Storage Vials (3 vials)
Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.

See our full line of Antibody & Protein Labeling Kits.

CF® Dye & Biotin SE Protein Labeling Kits

SE Protein Labeling KitEx/EmSizeCatalog No.Dye Features
CF®350347/448 nm3 x 1 mg labelings92210CF®350 Features
CF®405S404/431 nm3 x 1 mg labelings92211CF®405S Features
CF®405M408/452 nm3 x 1 mg labelings92212CF®405M Features
CF®405L395/545 nm3 x 1 mg labelings92228CF®405L Features
CF®488A490/515 nm3 x 1 mg labelings92213CF®488A Features
CF®532527/558 nm3 x 1 mg labelings92208CF®532 Features
CF®543541/560 nm3 x 1 mg labelings92209CF®543 Features
CF®555555/565 nm3 x 1 mg labelings92214CF®555 Features
CF®568562/583 nm3 x 1 mg labelings92215CF®568 Features
CF®594593/614 nm3 x 1 mg labelings92216CF®594 Features
CF®633630/650 nm3 x 1 mg labelings92217CF®633 Features
CF®640R642/662 nm3 x 1 mg labelings92225CF®640R Features
CF®647650/665 nm3 x 1 mg labelings92218CF®647 Features
CF®660R663/682 nm3 x 1 mg labelings92219CF®660R Features
CF®660C667/685 nm3 x 1 mg labelings92223CF®660C Features
CF®680681/698 nm3 x 1 mg labelings92220CF®680 Features
CF®680R680/701 nm3 x 1 mg labelings92226CF®680R Features
CF®750755/777 nm3 x 1 mg labelings92221CF®750 Features
CF®770770/797 nm3 x 1 mg labelings92222CF®770 Features
BiotinN/A3 x 1 mg labelings92224

Antibody & Protein Labeling Kits

Product
Name		Label
Options		Labeling
Scale		Labeling
Time		Features
Mix-n-Stain™ CF® Dye
Antibody Labeling Kits		CF® Dyes≤5-20 ug IgG
20-50 ug IgG
50-100 ug IgG		~ 15 min.• Rapid, simple labeling

• No purification

• Tolerates BSA & other additives

• Dyes for super-resolution, spectral flow, & NIR detection
Mix-n-Stain™ FITC
Antibody Labeling Kits		FITC
Mix-n-Stain™ Cyanine Dye
Antibody Labeling Kits		Cyanine 555
Cyanine 647
Mix-n-Stain™ Biotin
Antibody Labeling Kits		Biotin
Mix-n-Stain™ Digoxigenin
Antibody Labeling Kits		Digoxygenin (DIG)
Mix-n-Stain™ DNP
Antibody Labeling Kits		DNP
Mix-n-Stain™ Maxi
Antibody Labeling Kits		CF® Dyes
Cyanine Dyes		1 mg IgG~ 30 min.
Mix-n-Stain™ CF® Dye IgM Antibody Labeling KitsCF® Dyes
FITC		25 ug IgM
100 ug IgM		15-30 min.• Rapid, simple labeling

• No purification
Mix-n-Stain™ STORM CF® Dye Antibody Labeling KitsCF® Dyes50 ug IgG~ 30 min.• Optimized to provide low 1-2.5 DOL

• No purification
Mix-n-Stain™
Nanobody Labeling Kits		CF® Dyes5-20 ug Nanobody®
20-50 ug Nanobody®		~ 30 min.• Optimized for Nanobodies®

• Tolerates BSA & other additives
Mix-n-Stain™
Small Ligand Labeling Kits		CF® Dyes0.1 umol
small ligand		~ 30 min.• Label SNAP®, CLIP™, HaloTag® & other small ligands

• Dye options for surface or intracellular targets
VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo ImagingNear-IR CF® Dyes3 x 1 mg IgG~ 2 hours• Dyes, buffers, & spin vials for labeling + purification

• Sterile syringes, filters and storage vials for in vivo use
CF® Dye & Biotin SE
Protein Labeling Kits		CF® Dyes
Biotin		3 x 1 mg
protein		~ 2 hours• Dyes, buffers, & spin vials for labeling + purification

References

Download a list of CF® dye references.

 

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