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CF® Dye & Biotin SE Protein Labeling Kits

Kits for succinimidyl ester labeling antibodies or other proteins with Biotium’s next-generation CF® dyes or biotin. 3 x 1 mg labeling reactions per kit.

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3 labelings
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Product Description

This kit contains reagents for labeling antibodies or other proteins with a fluorescent CF® dye succinimidyl ester (CF® dye SE) or biotin succinimidyl ester (biotin SE). Learn more about next-generation CF® dyes with superior brightness, photostability, and water solubility compared to other fluorescent dyes.

Features

  • 3 labeling reactions of 1 mg each
  • Choice of biotin or CF® dyes ranging from UV to near-IR
  • Includes buffers & ultrafiltration vials for labeling & purification

Kit Components

  • Lyophilized CF® Dye SE or Biotin SE (3 vials)
  • Anhydrous DMSO
  • Sodium Bicarbonate Solution, pH 8.3
  • 1X PBS, pH 7.4
  • Ultrafiltration Vials, 10K MWCO (3 vials)
  • Reaction Vials (3 vials)
  • Storage Vials (3 vials)
Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.

See our full line of Antibody & Protein Labeling Kits.

CF® Dye & Biotin SE Protein Labeling Kits

SE Protein Labeling KitEx/EmSizeCatalog No.Dye Features
CF®350347/448 nm3 x 1 mg labelings92210CF®350 Features
CF®405S404/431 nm3 x 1 mg labelings92211CF®405S Features
CF®405M408/452 nm3 x 1 mg labelings92212CF®405M Features
CF®405L395/545 nm3 x 1 mg labelings92228CF®405L Features
CF®488A490/515 nm3 x 1 mg labelings92213CF®488A Features
CF®532527/558 nm3 x 1 mg labelings92208CF®532 Features
CF®543541/560 nm3 x 1 mg labelings92209CF®543 Features
CF®555555/565 nm3 x 1 mg labelings92214CF®555 Features
CF®568562/583 nm3 x 1 mg labelings92215CF®568 Features
CF®594593/614 nm3 x 1 mg labelings92216CF®594 Features
CF®633630/650 nm3 x 1 mg labelings92217CF®633 Features
CF®640R642/662 nm3 x 1 mg labelings92225CF®640R Features
CF®647650/665 nm3 x 1 mg labelings92218CF®647 Features
CF®660R663/682 nm3 x 1 mg labelings92219CF®660R Features
CF®660C667/685 nm3 x 1 mg labelings92223CF®660C Features
CF®680681/698 nm3 x 1 mg labelings92220CF®680 Features
CF®680R680/701 nm3 x 1 mg labelings92226CF®680R Features
CF®750755/777 nm3 x 1 mg labelings92221CF®750 Features
CF®770770/797 nm3 x 1 mg labelings92222CF®770 Features
BiotinN/A3 x 1 mg labelings92224

Antibody & Protein Labeling Kits

Product
Name
Label
Options
Labeling
Scale
Labeling
Time
Features
Mix-n-Stain™ CF® Dye
Antibody Labeling Kits
CF® Dyes≤5-20 ug IgG
20-50 ug IgG
50-100 ug IgG
~ 15 min.• Rapid, simple labeling

• No purification

• Tolerates BSA & other additives

• Dyes for super-resolution, spectral flow, & NIR detection
Mix-n-Stain™ FITC
Antibody Labeling Kits
FITC
Mix-n-Stain™ Cyanine Dye
Antibody Labeling Kits
Cyanine 555
Cyanine 647
Mix-n-Stain™ Biotin
Antibody Labeling Kits
Biotin
Mix-n-Stain™ Digoxigenin
Antibody Labeling Kits
Digoxygenin (DIG)
Mix-n-Stain™ DNP
Antibody Labeling Kits
DNP
Mix-n-Stain™ Maxi
Antibody Labeling Kits
CF® Dyes
Cyanine Dyes
1 mg IgG~ 30 min.
Mix-n-Stain™ CF® Dye IgM Antibody Labeling KitsCF® Dyes
FITC
25 ug IgM
100 ug IgM
15-30 min.• Rapid, simple labeling

• No purification
Mix-n-Stain™ STORM CF® Dye Antibody Labeling KitsCF® Dyes50 ug IgG~ 30 min.• Optimized to provide low 1-2.5 DOL

• No purification
Mix-n-Stain™
Nanobody Labeling Kits
CF® Dyes5-20 ug Nanobody®
20-50 ug Nanobody®
~ 30 min.• Optimized for Nanobodies®

• Tolerates BSA & other additives
Mix-n-Stain™
Small Ligand Labeling Kits
CF® Dyes0.1 umol
small ligand
~ 30 min.• Label SNAP®, CLIP™, HaloTag® & other small ligands

• Dye options for surface or intracellular targets
VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo ImagingNear-IR CF® Dyes3 x 1 mg IgG~ 2 hours• Dyes, buffers, & spin vials for labeling + purification

• Sterile syringes, filters and storage vials for in vivo use
CF® Dye & Biotin SE
Protein Labeling Kits
CF® Dyes
Biotin
3 x 1 mg
protein
~ 2 hours• Dyes, buffers, & spin vials for labeling + purification

References

Download a list of CF® dye references.

 

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