Biotin azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with phosphine containing compound.
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CF® Dye & Biotin SE Protein Labeling Kits
This kit contains everything you need to label antibodies or other proteins with a fluorescent CF® dye succinimidyl ester (CF® dye SE) or biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labeling reactions of up to 1 mg protein per reaction.
CF® Dye BCN
CF® dye with BCN reacts with azide to form 1,2,3-triazole by copper-free 1,3-dipolar Huisgen cycloaddition. This copper-free bioorthogonal reaction allows staining the surface of live cells or cell extracts when there are concerns about native protein function loss with copper. We also offer membrane-permeant CF® dye BCN options for intracellular reaction.
Streptavidin conjugates are prepared from high quality streptavidin biotin binding protein and Biotium's signature bright and photostable fluorescent CF® dyes, and a selection of other labels.
Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.
CF® Dye Alkyne
CF® dye alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It can also be used as a monomer to copolymerize with other monomers to form fluorescent polymers.