Biotin azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with phosphine containing compound.
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CF® Dye & Biotin SE Protein Labeling Kits
This kit contains everything you need to label antibodies or other proteins with a fluorescent CF® dye succinimidyl ester (CF® dye SE) or biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labeling reactions of up to 1 mg protein per reaction.
CF® Dye BCN
CF® dye with BCN reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper-free bioorthogonal reaction allows staining the surface of live cells or cell extracts when there are concerns about native protein function loss with copper.
Streptavidin conjugates are prepared from high quality streptavidin biotin binding protein and Biotium's signature bright and photostable fluorescent CF® dyes, and a selection of other labels.
Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.
CF® Dye Alkyne
CF® dye alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It can also be used as a monomer to copolymerize with other monomers to form fluorescent polymers.