Bovine Serum Albumin Fraction V
Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins and enzymes, including dilute solutions of antibody and use as a blocking agent to reduce non-specific binding. This BSA is protease- and IgG-free.
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Tween® 20 (polysorbate 20) is a polysorbate surfactant commonly used as a blocking and washing agent in Western blotting and other immunostaining assays. Tween® 20 is typically included in blocking buffers and washing buffers at a concentration of 0.1% to reduce non-specific antibody binding.
Bovine Serum Albumin 30% solution
Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins such as antibodies and enzymes, and as a blocking agent to reduce non-specific protein binding in immunoassays such as ELISA, immunofluorescence staining, and Western blotting. This is a 30% solution of BSA in water, prepared using IgG- and protease-free BSA.
10X Fish Gelatin Blocking Agent
10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.
Fish Gelatin Powder
Fish gelatin is commonly used in blocking solutions to minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Because this gelatin is derived from the skin of cold water fish it does not cross-react with mammalian antibodies.
Dry Milk Powder
Nonfat dry milk is commonly added to blocking buffers minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Milk can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.