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Fura-2, Pentapotassium Salt

Fura-2, pentapotassium salt is a membrane-impermeant ratiometric calcium indicator. It can be loaded into cells via microinjection or scrape loading.

Product Attributes

CAS number

113694-64-7

Applications

Calcium indicators

Cell permeability

Membrane impermeant

Indicator type

Ratiometric

Size
Catalog #
price
Qty
1 mg
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Product Description

Fura-2, pentapotassium salt is a ratiometric fluorescent dye that binds to free intracellular calcium. It is membrane-impermeant and may be loaded into cells via microinjection or scrape loading.

Fura-2 fluorescence can be detected using flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2+-free) to 335 nm (Ca2+-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels.

  • Light yellow solid soluble in DMSO
  • Store at 4°C and protect from light, especially in solution
  • Kd: 145 nM (22°C in pH 7.2 buffer)
  • λExEm (no Ca2+) = 363/512 nm; λExEm (high Ca2+) = 335/505 nm
  • ε (363 nm, no Ca2+) = 27,000 M-1cm-1; ε (335 nm, high Ca2+) = 35,000 M-1cm-1
  • C29H22K5N3O14
  • MW: 832
  • [113694-64-7]

 

 

Fura-2 Products

Product

Unit Size

Catalog Number

Fura-2, pentaammonium salt1 mg50030
Fura-2, pentapotassium salt1 mg50031
Fura-2, pentasodium salt1 mg50032
Fura-2, AM ester10 x 100 ug50033
20 x 50 ug50033-1
1 mg50034
Fura-2, AM ester, 1 mM solution1 mL50029

References

1. Science

248, 73 (1990).

 

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