One-Step Lumitein™ Protein Gel Stain

One-Step Lumitein™ Protein Gel Stain is a ready-to-use luminescent red protein gel stain. It is a dramatically improved version of our original Lumitein™ protein gel stain in terms of convenience and safety. One-Step Lumitein™ can detect as little as approximately 1-10 ng of protein per band.

• One-step staining procedure, no fixation required
• Non-toxic formulation is safe for easy disposal
• Sensitive protein detection down to 1-10 ng
• Image with a UV transilluminator, a blue light transilluminator, or laser-based gel scanner
• 1 L and 4 L sizes
• Store at 4°C

Fast and simple procedure

One-Step Lumitein™ gel staining requires only a single 5-60 minute staining step without fixation. Destaining is optional. One-Step Lumitein™ can detect as little as approximately 1-10 ng of protein per band depending on the staining method used, although staining intensity varies between proteins. Staining is fully compatible with mass spectrometry and Edman-based sequencing. Staining can be imaged using a UV transilluminator with EtBr or SYBR filter set, a blue light transilluminator, or a laser-based gel scanner.

One-Step Lumitein™ is non-toxic

One-Step Lumitein™ Protein Gel Stain offers safer handling and ease of disposal compared to other protein gel stains, because it is an aqueous-based solution that does not contain hazardous methanol or acetic acid. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step. Furthermore, One-Step Lumitein™ costs significantly less than other fluorescent protein gel stains. One-Step Lumitein™ Protein Gel Stain is available in 1 liter bottle or 4 liter Cubitainer® sizes.

Red fluorescent One-Step Lumitein™ is an alternative to time-consuming and expensive SYPRO® Ruby protein gel stain, and it can be imaged on a UV box or fluorescence gel scanner

Please visit our Protein Gel Stains technology page to learn about our other safe and sensitive protein gel stains such as One-Step Lumitein™ UV, which is optimized for use with a UV transilluminator, and One-Step Blue™, a rapid, easy-to-use, non-toxic alternative to Coomassie staining for visible blue staining.