Support & Resources

+1

VersaBlot™ Total Protein Normalization Kits

VersaBlot™ Total Protein Normalization Kits are designed for reversible protein visualization and quantitation on western blot membranes via protein labeling with near-infrared CF® dyes. The labeling shows superior linearity for western normalization compared to housekeeping proteins.

Dye Color
Size
Catalog #
price
Qty
100 labelings
500 labelings
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Protocols
Supporting Documents

Product Description

VersaBlot™ Total Protein Normalization Kits are designed for reversible, sensitive and highly linear protein quantification on SDS-PAGE gels and western blot membranes.

Features

  • Reversible protein prestain for fluorescent western blot normalization
  • Superior linearity for western blot normalization compared to housekeeping proteins
  • Highly sensitive and accurate, detect as little as 1 ng protein and 10% difference in protein content
  • Fast and easy labeling of proteins or cell lysates with near-IR CF® dyes before SDS-PAGE
  • CF®680T (Ex/Em 681/698 nm) for Typhoon™ Cy®5 or IR Short filter or Odyssey® 700 channel
  • CF®770T (Ex/Em 764/787 nm) for Typhoon™ IR Long filter or Odyssey® 800 channel

VersaBlot™ Total Protein Normalization Kits are designed for rapid and sensitive protein detection on SDS-PAGE gels and western blot (WB) membranes. The proteins are covalently labeled with near-infrared CF® Dyes so that after electrophoresis, the bands can be directly visualized via fluorescent gel scanning, eliminating the need for any gel staining procedures. The labeled proteins on SDS-PAGE gels can then be transferred to membranes for WB normalization. If desired, the prestain can be reversed after scanning, facilitating downstream multi-color WB using antibodies conjugated to near-infrared CF® Dyes in the same channel (see Protocol Overview below).

For SDS-PAGE gel prestaining, the kits allow detection of low concentration proteins down to 1 ng with minimal background. The dyes do not cause any visible change to the shape or mobility of the bands compared to unlabeled proteins visualized by post-staining methods.

For WB normalization, the kits demonstrate outstanding linearity for quantification of total protein contents over a wide dynamic range (0.1 – 15 ug), outperforming the traditional normalization method based on housekeeping proteins. Compared to total protein post-staining products like the Ponceau S and LI-COR® REVERT™ Total Protein Stain, VersaBlot™ features a prestaining protocol with superior signal-to-noise and sensitivity (able to detect 10% difference in protein content), with comparable reversibility and ease-of-use. The labeling and reversal procedures do not affect protein mobility or detection by antibodies (including phosphoprotein and glycoprotein detection).

Choosing the Right Dye

The VersaBlot™ Total Protein Normalization Kits enable multi-color protein detection on membranes, with or without reversal. It is important to maintain balanced signal intensity across all channels for multi-color imaging (i.e. not having one color dramatically brighter than others). If signal crosstalk is a concern, we recommend to use the VersaBlot™ CF®770T Total Protein Normalization Kit.

See our fluorescent western blotting protocols.

Protocol Overview

 

REVERT is a registered trademark of LI-COR Biosciences.

 

You may also like…