One-Step Lumitein™ Protein Gel Stain is a ready-to-use luminescent protein gel stain. It is a dramatically improved version of our original Lumitein™ protein gel stain for convenience and safety. One-Step Lumitein™ gel staining requires only a single 5-60 minute staining step without fixation. Destaining is optional. Moreover, One-Step Lumitein™ Protein Gel Stain offers safer handling and ease of disposal, because it is an aqueous-based solution that does not contain hazardous methanol or acetic acid. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step. The One-Step stains are compatible with Edman-based sequencing and with mass spectrometry. Furthermore, One-Step Lumitein™ costs significantly less than other fluorescent protein gel stains. One-Step Lumitein™ Protein Gel Stain is available in 1 liter bottle or 4 liter Cubitainer® sizes.
One-Step Lumitein™ can detect as little as approximately 1-10 ng of protein per band depending on the staining method used, although staining intensity varies between proteins. Staining is fully compatible with mass spectrometry and Edman-based sequencing.
Biotium also offers One-Step Lumitein™ UV Protein Gel Stain, which is optimized for use with a UV transilluminator, but is not compatible with a blue light illuminator or laser-based scanner.
Also see our One-Step Blue™ Protein Gel Stain, a rapid, easy-to-use, non-toxic alternative to Coomassie staining for visible blue protein staining and optional near-infrared fluorescence-based gel imaging.