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One-Step Lumitein™ Protein Gel Stain

One-Step Lumitein™ Protein Gel Stain is a ready-to-use luminescent protein gel stain. It is a dramatically improved version of our original Lumitein™ protein gel stain for convenience and safety.

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Product Description

One-Step Lumitein™ Protein Gel Stain is a ready-to-use luminescent red protein gel stain. It is a dramatically improved version of our original Lumitein™ protein gel stain for convenience and safety. One-Step Lumitein™ gel staining requires only a single 5-60 minute staining step without fixation. Destaining is optional. One-Step Lumitein™ can detect as little as approximately 1-10 ng of protein per band depending on the staining method used, although staining intensity varies between proteins. Staining is fully compatible with mass spectrometry and Edman-based sequencing. Staining can be imaged using a UV transilluminator with EtBr or SYBR filter set, a blue light transilluminator, or a laser-based gel scanner.

One-Step Lumitein™ Protein Gel Stain offers safer handling and ease of disposal compared to other protein gel stains, because it is an aqueous-based solution that does not contain hazardous methanol or acetic acid. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step. Furthermore, One-Step Lumitein™ costs significantly less than other fluorescent protein gel stains. One-Step Lumitein™ Protein Gel Stain is available in 1 liter bottle or 4 liter Cubitainer® sizes.

Biotium also offers One-Step Lumitein™ UV Protein Gel Stain, which is optimized for use with a UV transilluminator, but is not compatible with a blue light illuminator or laser-based scanner.

Also see our One-Step Blue™ Protein Gel Stain, a rapid, easy-to-use, non-toxic alternative to Coomassie staining for visible blue protein staining and optional near-infrared fluorescence-based gel imaging.

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Support & Faq

Protein Gel Stains

Use of pre-stained protein markers can quench lumitein fluorescence. Switching to an unstained marker should resolve the issue.

Category: Protein Gel Stains

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Lumitein™ is a luminescent dye designed for detecting proteins in SDS polyacrylamide (SDS-PAGE) gels. It can also be used to detect proteins in native PAGE gels after an additional SDS incubation step as described in the protocol (PI-21002).

Category: Protein Gel Stains

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