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One-Step Lumitein™ Protein Gel Stain

One-Step Lumitein™ Protein Gel Stain is a ready-to-use luminescent protein gel stain. It is a dramatically improved version of our original Lumitein™ protein gel stain for convenience and safety.

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Product Description

One-Step Lumitein™ Protein Gel Stain is a ready-to-use luminescent red protein gel stain. It is a dramatically improved version of our original Lumitein™ protein gel stain for convenience and safety. One-Step Lumitein™ gel staining requires only a single 5-60 minute staining step without fixation. Destaining is optional. One-Step Lumitein™ can detect as little as approximately 1-10 ng of protein per band depending on the staining method used, although staining intensity varies between proteins. Staining is fully compatible with mass spectrometry and Edman-based sequencing. Staining can be imaged using a UV transilluminator with EtBr or SYBR filter set, a blue light transilluminator, or a laser-based gel scanner.

One-Step Lumitein™ Protein Gel Stain offers safer handling and ease of disposal compared to other protein gel stains, because it is an aqueous-based solution that does not contain hazardous methanol or acetic acid. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step. Furthermore, One-Step Lumitein™ costs significantly less than other fluorescent protein gel stains. One-Step Lumitein™ Protein Gel Stain is available in 1 liter bottle or 4 liter Cubitainer® sizes.

Biotium also offers One-Step Lumitein™ UV Protein Gel Stain, which is optimized for use with a UV transilluminator, but is not compatible with a blue light illuminator or laser-based scanner.

Also see our One-Step Blue™ Protein Gel Stain, a rapid, easy-to-use, non-toxic alternative to Coomassie staining for visible blue protein staining and optional near-infrared fluorescence-based gel imaging.

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Support & Faq

Protein Gel Stains

Use of pre-stained protein markers can quench lumitein fluorescence. Switching to an unstained marker should resolve the issue.

Category: Protein Gel Stains

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Lumitein™ is a luminescent dye designed for detecting proteins in SDS polyacrylamide (SDS-PAGE) gels. It can also be used to detect proteins in native PAGE gels after an additional SDS incubation step as described in the protocol (PI-21002).

Category: Protein Gel Stains

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Shipping & Handling Disclaimer: Shipping and handling methods are calculated based on item temperature storage recommendation. Large, multi-item orders may be assessed additional fees. Shipping to Puerto Rico may be assessed additional fees. A Biotium customer service representative will contact you if additional fees are required. For expedited shipping, please request in the Order Notes section of the checkout page. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.